中国中医药信息杂志Issue(1)：47-49,3.DOI:10.3969/j.issn.1005-5304.2015.01.014
红芪黄酮对肺纤维化模型大鼠基质金属蛋白酶-2及其抑制剂-1蛋白表达的影响
Effects of Radix Hedysari Flavonoids on Expressions of MMP-2 and TIMP-1 Protein in Rat Pulmonary Fibrosis Model
摘要
Abstract
Objective To discuss radix hedysari flavonoids mechanism of preventing pulmonary fibrosis.Methods Totally 216 SPF Wistar rats were randomized into normal group, model group, prednisone group, radix hedysari flavonoids high, medium, and low dose groups, and then pulmonary fibrosis model was established by intratracheal dripping of bleomycin. From the second day after modeling, every treatment group received gavage with the corresponding dose of medicine at the planned time for 7, 14, and 28 continuous days. Expressions of MMP-2 and TIMP-1 protein were detected by immunohistochemical method.Results When radix hedysari flavonoids high dose group was at the 7th, 14th, 28th day, and medium dose group was at the 14th day, MMP-2 protein expression was lower than model group, similar to prednisone group. When radix hedysari flavonoids high dose group was at the 14th, 28th day and medium dose group was at 14th day, TIMP-1 protein expression was lower than model group.Conclusion Radix hedysari flavonoids can adjust the expressions of MMP-2 and TIMP-1 protein at different phases, and tend to make the balance of MMPs/TIMPs, which may be an effective mechanism for the inhibition of fibrosis process.关键词
肺纤维化/红芪黄酮/基质金属蛋白酶-2/基质金属蛋白酶抑制剂-1/大鼠Key words
pulmonary fibrosis/radix hedysari flavonoids/MMP-2/TIMP-1/rats分类
医药卫生引用本文复制引用
苏韫,张毅,李娟,刘光炜,李雪燕,蔺兴遥,李金田,景明..红芪黄酮对肺纤维化模型大鼠基质金属蛋白酶-2及其抑制剂-1蛋白表达的影响[J].中国中医药信息杂志,2015,(1):47-49,3.基金项目
国家自然科学基金地区基金(30960037/C020409)；教育部春晖计划项目(Z2004-1-620039)；甘肃中医学院中青年基金(2010-2) （30960037/C020409）
