中国中医药信息杂志Issue(1)：67-69,3.DOI:10.3969/j.issn.1005-5304.2015.01.019
不同浓度血通灵对人乳腺癌细胞侵袭作用及基质金属蛋白酶-9蛋白表达的影响
Effects of Chinese FormulaXuetongling on MDA-MB-231 Human Breast Cancer Cell Invasion and MMP-9 Protein Expression
姚蓝 1马红梅 2杜冰洁 3李在宪 1崔征1
作者信息
- 1. 新疆医科大学中医学院，新疆 乌鲁木齐 830011
- 2. 东亚大学，韩国 釜山 604714
- 3. 沈阳药科大学，辽宁 沈阳 110016
- 折叠
摘要
Abstract
Objective To research the action mechanism of Chinese FormulaXuetongling on MDA-MB-231 human breast cancer cell invasion.Methods Transwell Invasion assay was applied to investigate the inhibitory effects on cancer cell invasion ofXuetongling extract in different concentrations;MMP-9 secretion activity was detected by zymography assay after the treatment of XTL extract in MDA-MB-231;Western blot was used to detect the effect of XTL extract on MMP-9 protein expression in MDA-MB-231.ResultsXuetongling extract in different concentrations significantly suppressed the cell invasion, MMP-9 secretion and MMP-9 protein expression in dose dependent manner.Conclusion The inhibitory effect ofXuetongling on MDA-MB-231 cell invasion may be due to the down-regulation of both MMP-9 secretion and MMP-9 protein expression.关键词
血通灵/人乳腺癌细胞/侵袭作用/基质金属蛋白酶-9Key words
Xuetongling/MDA-MB-231/invasion assay/MMP-9分类
医药卫生引用本文复制引用
姚蓝,马红梅,杜冰洁,李在宪,崔征..不同浓度血通灵对人乳腺癌细胞侵袭作用及基质金属蛋白酶-9蛋白表达的影响[J].中国中医药信息杂志,2015,(1):67-69,3.
