中国中医药信息杂志Issue(1)：87-90,4.DOI:10.3969/j.issn.1005-5304.2015.01.025
清咽颗粒质量标准研究
A Quality Standard Study onQingyan Granule
摘要
Abstract
Objective To establish the standard for quality control ofQingyan Granule. Methods The chief components of the preparation, Sophora Tonkinensis radix et rhizoma, Adenophorae radix, Lonicera japonica caulis, and Ophiopogonis radix were identified by TLC qualitatively. The contents of licorice glycosides and glycyrrhizic acid were determined by HPLC. The separation was performed on Thermo Syncronis C18 column (4.6 mm×250 mm, 5μm) with mobile phase consisted of acetonitrile with 0.05% phosphoric acid solution (A)-0.05% phosphoric acid solution (B), and gradient elution (0-8 min, 19%A;8-35 min, 19%→50%A). Detection wavelength was 237 nm, and flow rate was 1 mL/min.Results The spots in TLC were clear. There were spots with same color on the corresponding location of reference substance and reference herbal, negative control without interference. The linear range for licorice glycosides was 0.05-0.5μg (r=0.999 9). The average recovery was 99.97%, RSD=1.74% (n=9). The linear range for glycyrrhizic acid was 0.1-2μg (r=0.999 9). The average recovery was 99.74%, RSD=1.28% (n=9). Conclusion The method is simple, accurate, with high reproducibility, which can be used for quality control ofQingyan Granule.关键词
清咽颗粒/甘草苷/甘草酸铵/薄层色谱法/高效液相色谱法/含量测定Key words
Qingyan Granula/licorice glycosides/ammonium glycyrrhetate/TLC/HPLC/content determination分类
医药卫生引用本文复制引用
马燕,李明春,付延伟,张华,程艳芹..清咽颗粒质量标准研究[J].中国中医药信息杂志,2015,(1):87-90,4.基金项目
军队医疗机构制剂标准提高科研专项(13ZJZ03) （13ZJZ03）
