中国中医药信息杂志Issue(2)：47-50,4.DOI:10.3969/j.issn.1005-5304.2015.02.014
新疆塔里木马鹿茸多肽对小鼠前成骨细胞的影响
Effect of Antler Peptides of Cervus Elaphus Yarkandensis on MC3T3-E1 Cells
摘要
Abstract
Objective To evaluate the effect of antler polypeptides from Cervus elaphus yarkandensis on MC3T3-E1 cells. Methods The concentration of antler polypeptides of Cervus elaphus yarkandensis was measured by BCA protein assay kit. MC3T3-E1 cells were cultured and analyzed by BCIP/NBT chromogenic alkaline phosphatase (ALP) staining kit. After being induced to form mineralized knot, the cells were stained by using Alizarin red staining. Three different concentrations (10, 1.0, 0.1 μg/mL) of antler polypeptides were analyzed by MTT method and micronutrients enzymes standard method to determine the effect of cell proliferation and ALP synthesis. Results The concentration of antler polypeptides was 0.07 mg/mL. The results of in vitro cell activity analysis showed that the positive rate of ALP was 90%and the mineralization knot was stained red. Compared with the control group, the different concentrations of antler polypeptides all showed the function of cell proliferation and the effect was dose-dependent after 3 d and 7 d. Compared with the control group, at the 3 d, three groups of antler polypeptides promoted synthesis and secretion of ALP (P<0.01) and the results showed a dose-dependent effect. Conclusion Antler polypeptides could obviously promote the proliferation of MC3T3-E1 cells and secretion of ALP, which indicated that antler polypeptides have certain effect on osteoporosis.关键词
塔里木马鹿/鹿茸多肽/成骨细胞/细胞增殖/碱性磷酸酶/小鼠Key words
Cervus elaphus yarkandensis/antler polypeptides/osteoblasts/cell proliferation/alkaline phosphatase/mice分类
医药卫生引用本文复制引用
吕秀华,陈伟,周凡,刘斐,武嘉林..新疆塔里木马鹿茸多肽对小鼠前成骨细胞的影响[J].中国中医药信息杂志,2015,(2):47-50,4.基金项目
国家科技支撑计划(2011BAI05B03) （2011BAI05B03）
