中国中医药信息杂志Issue(2)：79-82,4.DOI:10.3969/j.issn.1005-5304.2015.02.022
不同黄芪和党参栽培品种（系）遗传关系的ISSR分析
Analysis of Genetic Relationship of Astragalus membranaceus and Codonopsis pilosula from Different Cultivars (Varieties) by ISSR
摘要
Abstract
Objective To investigate the genetic relationships of Astragalus membranaceus and Codonopsis pilosula from the different cultivars (varieties). Methods Twenty-five samples of A. membranaceus and seventeen samples of C. Pilosula were amplified by ISSR-PCR. Genetic diversity and genetic distance were analyzed by Popgen32. UPGMA dendrogram relationship was clustered by NTSYS. Results Totally 62 and 100 loci were detected respectively from A. membranaceus and C. pilosula. All percentages of polymorphic loci (PPL) were 100%. Shannon’s information indexes (I) were 0.537 6 and 0.472 7. Nei’s genetic diversity indexes (H) were 0.361 3 and 0.307 4. A. membranaceus was divided into two groups in genetic distance 0.59, and C. pilosula was alike in 0.50. Conclusion Different cultivars (varieties) of A. membranaceus and C. pilosula had rich genetic diversities among species, and had large genetic differences.关键词
黄芪/党参/ISSR分子标记技术/遗传关系Key words
Astragalus membranaceus/Codonopsis pilosula/ISSR/genetic relationship分类
医药卫生引用本文复制引用
朱田田,晋玲,刘效瑞,杜弢,李应东,张裴斯..不同黄芪和党参栽培品种（系）遗传关系的ISSR分析[J].中国中医药信息杂志,2015,(2):79-82,4.基金项目
国家科技支撑计划(2011BAI05B02)；国家自然科学基金(81360615)；甘肃省自然科学基金(1308RJZA127) （2011BAI05B02）
