中国中医药信息杂志Issue(2)：86-90,5.DOI:10.3969/j.issn.1005-5304.2015.02.024
栀子叶绿体DNA条形码的研究
Identification of Gardenia Based on cpDNA Barcoding
摘要
Abstract
Objective To test and eva1uate the abi1ity of three potential chloroplast DNA (cpDNA) barcoding sequences;To find new methods to identify the species of gardenia. Methods Three cpDNA sequences were amplified and sequenced by universal primers of matK, rbcL and psbA. By comparing PCR amplification efficiency, length, intra-and inter-specific divergence, and barcoding gap, BLAST and DNA MAN were used to evaluate these loci. Results The amplification efficiency of 5 samples from 3 gardenia species was 100%. Analysis of the intra-and inter-specific divergence of matK among the sequences showed that barcoding gap was superior to psbA and rbcL, with higher identification efficiency. Conclusion Gardenia jasminoides Ellis can be better identified by matK sequence.关键词
叶绿体DNA条形码/栀子/marK/rbcL/psbAKey words
cpDNA barcoding/gardenia/matK/rbcL/psbA分类
医药卫生引用本文复制引用
伍美慧,鲁璨,崔培梧,鲁耀邦,李笑,罗娇..栀子叶绿体DNA条形码的研究[J].中国中医药信息杂志,2015,(2):86-90,5.基金项目
湖南省大学生创新基金(0003000100801743)；湖南省研究生教改课题(JG2011B031)；湖南省重点学科-中药学(2011年)；湖南中医药大学重点学科-生物工程(2012年) （0003000100801743）
