中国中医药信息杂志Issue(3)：51-55,5.DOI:10.3969/j.issn.1005-5304.2015.03.014
不同针刺刺激量对寒凝证类痛经大鼠子宫微循环及其相关物质的影响
Effect of Different Acupuncture Stimuli on Uterine Micro-circulation and Circulation-related Substances of Dysmenorrheal Rats with Cold Stagnation Syndrome
摘要
Abstract
Objective To explore the effect of different acupuncture stimuli on uterine micro-circulation of dysmenorrheal rats with cold stagnation syndrome. Methods Totally 32 three-month old female SD rats in diestrus were randomly divided into saline control group, model group, A stimuli group, and B stimuli group, 8 rats in each group. Model group and treatment groups were given whole body freezing combined with estradiol benzoate injection method to establish models. A stimuli group was given deep puncture with manipulation, while B stimuli group was treated by shallow puncture without manipulation. Diameter of uterine capillary,micro-vessel, TXB2, and 6-keto-PGF1αlevels were observed in each group. Results Compared with the saline group, capillary diameter in model group was significantly reduced at 5, 10, 20, 30 min time point (P<0.01);micro-vascular diameter was significantly reduced at 5, 10, 20, 30, 40 min time point (P<0.01);plasma 6-keto-PGF1α levels decreased (P<0.01);TXB2/6-keto-PGF1αincreased significantly (P<0.01). Compared with model group, capillary diameter in A stimuli group enlarged at 5, 10, 20, 30 min time point (P<0.05), micro-vascular diameter dilated at 5, 10, 20, 30, 40 min time point (P <0.01), plasma 6-keto-PGF1α level increased (P <0.05), TXB2/6-keto-PGF1αdecreased significantly (P<0.05);micro-vascular diameter in B stimuli group dilated at 20, 30 min time point (P<0.05), plasma TXB2/6-keto-PGF1α decreased significantly (P<0.05). Compared with B stimuli group, capillary diameter in A stimuli group dilated at 5, 10, 20, 30 min time point (P<0.05) and micro-vascular diameter dilated at 5, 10, 20, 30, 40 min time point significantly (P<0.01). Conclusion Dysmenorrheal rats with cold stagnation syndrome show obvious disorder of the uterus micro-circulation and circulation related substances. Both A and B acupuncture stimuli improved uterus micro-circulation of dysmenorrheal rats with cold stagnation syndrome, and its mechanism may be related to the recovery the balance between TXB2 and 6-keto-PGF1α.关键词
不同针刺刺激量/寒凝证类痛经大鼠/子宫微循环/血栓素B2/6-酮-前列腺素-F1αKey words
different acupuncture stimuli/dysmenorrheal rats with cold stagnation syndrome/uterine micro-circulation/TXB2/6-keto-PGF1α分类
医药卫生引用本文复制引用
章庆庆,许咏思,王洋,赵雅芳,嵇波,任晓暄,张露芬,朱江,李晓泓,朱世鹏,罗丽,郭孟玮,张玲,申松希,齐丹丹,杨佳敏,沈小雨..不同针刺刺激量对寒凝证类痛经大鼠子宫微循环及其相关物质的影响[J].中国中医药信息杂志,2015,(3):51-55,5.基金项目
国家重点基础研究发展计划(2012CB518506、2006CB504503、2005CB523308)；教育部高等学校博士点科研基金(20090013110005)；国家自然科学基金面上项目(30973793) （2012CB518506、2006CB504503、2005CB523308）
