中国中医药信息杂志Issue(3)：80-82,3.DOI:10.3969/j.issn.1005-5304.2015.03.021
新疆阿魏抗氧化活性部位研究
Study on Anti-oxidant Activity Parts of Ferula sinkiangensis K. M. Shen
摘要
Abstract
Objective To establish a high-throughput detection of medicine on DPPH free radical scavenging activity;To analyze and preliminarily screen the anti-oxidant activity part of Ferula sinkiangensis. Methods Taking DPPH free radical scavenging rate of ascorbic acid as positive control, and IC50 as the evaluating criterion of DPPH free radical scavenging capability, oxidation resistance of different polar parts (petroleum ether part, ethyl acetate part, methyl alcohol part, and water part) of Ferula sinkiangensis was evaluated and screened. Results Different polarity parts of Ferula sinkiangensis DPPH radical scavenging of IC50 were:petroleum ether part 3252.22 μg/mL, ethyl acetate part 36.22 μg/mL, methyl alcohol part 32.22 μg/mL, water part 2643.38 μg/mL, and positive control group ascorbic acid 27.16 μg/mL. Conclusion The ethyl acetate and methyl alcohol parts of Ferula sinkiangensis were effective anti-oxidant activity site to eliminate DPPH free radicals. In this study, a simple, sensitive, and high-throughput detection method of DPPH radical scavenging assay was established to provide reference for the anti-oxidation medicine detection screening.关键词
新疆阿魏/1,1-二苯基-2-三硝基苯肼自由基/抗氧化活性/抗坏血酸Key words
Ferula sinkiangensis K. M. Shen/DPPH free radical/anti-oxidant activity/ascorbic acid分类
医药卫生引用本文复制引用
张海英,周龙龙,姜林,周铭心..新疆阿魏抗氧化活性部位研究[J].中国中医药信息杂志,2015,(3):80-82,3.基金项目
国家自然科学基金(81260625) （81260625）
