中国中医药信息杂志Issue(4)：47-50,4.DOI:10.3969/j.issn.1005-5304.2015.014
补肾活血方对卵巢早衰小鼠颗粒细胞凋亡的影响
Effects of Bushen Huoxue Prescription on Granulosa Cell Apoptosisi of Premature Ovarian Failure Mice
摘要
Abstract
Objective To discuss Bushen Huoxue Prescription mechanism of action on follicular granulosa cell apoptosis of premature ovarian failure (POF) mice. Methods The mouse zona pellucida 3 as the antigen, multi-point injection of subcutaneous immunization BALB/c to female mice was used to establish autoimmune POF model. Forty clean female mice were randomly divided into blank group, model group, Estradiol Valerate group and Bushen Huoxue Prescription group. All medicine groups were given relevant medicine for gavage, while model and blank groups were given normal saline with the same amount for gavage for 15 days. After the treatment, ovary, uterus, and thymus were weighed, and the viscera index was calculated. An ovarian slice was taken and HE staining was used to observe granulosa cell apoptosis. The levels of E2, LH, and FSH were detected by using the radioimmunoassay method. RT-PCR was used to analyze Fas and Fas-L mRNA expressions in ovarian granulosa cells. Results Compared with blank group, the weight of ovary, thymus, and uterus decreased significantly (P<0.05, P<0.01);Serum E2 level dropped (P<0.01);levels of FSH and LH, and expressions of Fas and Fas-L mRNA increased significantly (P<0.01). Compared with model group, granulosa cell apoptosis significantly decreased, and ovary index, uterus index, and thymus index increased (P<0.05);serum E2 level increased (P<0.01);levels of FSH and LH, and expressions of Fas and Fas-L mRNA significantly decreased (P<0.05). Conclusion Bushen Huoxue Prescription can decrease expressions of Fas and Fas-L mRNA to inhibit cell apoptosis by adjusting the hormone level of hypothalamus-pituitary-ovarianaxis.关键词
补肾活血方/卵巢早衰/颗粒细胞/细胞凋亡/小鼠Key words
Bushen Huoxue Prescription/premature ovarian failure/granulose cell/cell apoptosis/mice分类
医药卫生引用本文复制引用
刘慧萍,肖艺,李玲,祁鹏,宋焰,厉佳俊,易海青,柯超,张国民..补肾活血方对卵巢早衰小鼠颗粒细胞凋亡的影响[J].中国中医药信息杂志,2015,(4):47-50,4.基金项目
国家自然科学基金(81303123)；中国博士后科学基金(2013T60770、2012M511382)；高等学校博士学科点专项科研基金(20114323120007)；湖南省重点实验室开放基金(2014XNFZ06)；湖南省大学生创新训练中心资助项目(2013年)；湖南省重点学科-中西医结合基础(2012年)；湖南中医药大学重点学科-病理学及病理生理学(2012年) （81303123）
