中国中医药信息杂志Issue(4)：62-66,5.DOI:10.3969/j.issn.1005-5304.2015.018
冰片对HaCaT细胞膜流动性及膜电位的影响
Effects of Borneol on Membrane Fluidity and Membrane Potential of HaCaT Cell
摘要
Abstract
Objective To investigate the action mode of borneol on activity of epidermal skin;To investigate action mode of borneol as penetration enhancer. Methods The well-established and standard penetration enhancer Azone was employed as a positive control in this study. The cytotoxicities of borneol and Azone on HaCaT cells were detected by CCK-8 assay, and their half 50% inhibitory concentrations (IC50) were calculated. The fluorescence recovery after photo bleaching was employed to investigate the effect of borneol and Azone on membrane fluidity, and the flow cytometer was used to monitor the changes of membrane potential of HaCaT cell after treated with these penetration enhancers. Results The IC50 values of borneol and Azone were 2.826 , 0.172 mmol/L, respectively. Borneol could significantly improve the membrane fluidity in a concentration-dependent manner, and effectively decrease the membrane potential of HaCaT cell, which exhibited the performances similar to those of Azone. Conclusion The penetration enhancement mechanism of borneol was associated with the concentrations of Ca2+ in keratinocytes, which changes the membrane fluidity and membrane potential of HaCaT cell.关键词
冰片/促透剂/细胞膜流动性/HaCaT细胞/细胞膜电位Key words
borneol/penetration enhancer/membrane fluidity/HaCaT cell/membrane potential分类
医药卫生引用本文复制引用
傅大莉,雍小兰,刘德芳,肖爽..冰片对HaCaT细胞膜流动性及膜电位的影响[J].中国中医药信息杂志,2015,(4):62-66,5.基金项目
四川省卫生厅科研课题(120573) （120573）
