中国中医药信息杂志Issue(4)：83-86,4.DOI:10.3969/j.issn.1005-5304.2015.04.023
基于气相色谱-质谱技术的小青龙汤证大鼠血液代谢组学研究
Metabonomics Study on Blood of Rats with Xiaoqinglong Decoction Syndrome Based on GC-MS
摘要
Abstract
Objective To determine the endogenous metabolites in blood of rats with Xiaoqinglong Decoction Syndrome by using GC-MS;To provide evidence for researches on the essence of Xiaoqinglong Decoction Syndrome. Methods Totally 36 male Wistar rats were randomly divided into blank control group (10 rats), model group (13 rats), and Xiaoqinglong Decoction group (13 rats). Model group and Xiaoqinglong Decoction group were used to make the models of exogenous cold and internal fluid syndrome (Xiaoqinglong Decoction Syndrome). Rats in Xiaoqinglong Decoction group received gavage with Xiaoqinglong Decoction, while rats in blank control group received nothing. Blood of rats in the three groups was taken from the abdominal aorta and detected by GC-MS 15 days later, and analyzed by multivariate statistical analysis with PLS-DA. Results 13 kinds of biomarkers were found:lactic acid, 3-hydroxy-butyric acid, glycine, serine, lysine, dextrose, arachidonic acid and other metabolites. Conclusion Expressions of biomarkers, such as lactic acid and glycine, are energy metabolism, immune function, and inflammation, which provide material basis for researches on the essence of Xiaoqinglong Decoction Syndrome.关键词
气相色谱-质谱/血液/小青龙汤证/大鼠Key words
GC-MS/blood/Xiaoqinglong Decoction Syndrome/rats分类
医药卫生引用本文复制引用
赵小华,何丽清,盖江华..基于气相色谱-质谱技术的小青龙汤证大鼠血液代谢组学研究[J].中国中医药信息杂志,2015,(4):83-86,4.基金项目
国家自然科学基金(81102538)；山西中医学院临床基础项目(2011JC05) （81102538）
