中国中医药信息杂志Issue(5)：76-79,4.DOI:10.3969/j.issn.1005-5304.2015.05.021
清金化痰汤对慢性阻塞性肺疾病模型大鼠肺组织中性粒细胞弹性蛋白酶及黏蛋白基因表达的影响
Effects of Qingjin Huatan Decoction on Expressions of NE, EGFR, and MUC5AC mRNA in ;Lung Tissue of COPD Rats
摘要
Abstract
Objective To investigate the action mechanism of Qingjin Huatan Decoction on regulating COPD rats with mucus hypersecretion in airway. Methods Fifty 6-8 week old Wistar male rats were randomly divided into healthy group, model group, Qingjin Huatan Decoction group, clarithromycin group, 10 rats in each group. Except for healthy group, all the other groups were used the combination method of injecting LPS and smudging to establish COPD models. The last three groups were fed with normal saline, Qingjin Huatan Decoction, clarithromycin respectively for 30 days. On the 31st day of the experiment, the rats were put to death to take lung tissue. 6 rats from each group were chosen randomly, and the protein expressions of NE, EGFR and MUC5AC mRNA in lung tissue were detected by real-time fluorescent quantitative PCR. Results The expressions of NE, MUC5AC mRNA in lung tissue of COPD rats were higher than the healthy group. Compared with model group, the expressions of NE mRNA and MUC5AC mRNA in Qingjin Huatan Decoction group and clarithromycin group were markedly lower, while the expressions of NE, MUC5AC mRNA in Qingjin Huatan Decoction group were significantly lower than those in clarithromycin group. The expression of EGFR mRNA in Qingjin Huatan Decoction group was lower than that in model group. Conclusion Qingjin Huatan Decoction can inhibit mucus hypersecretion in airway through NE/EGFR/MUC5AC signal transduction pathway.关键词
清金化痰汤/慢性阻塞性肺疾病/黏液高分泌/黏蛋白/中性粒细胞弹性蛋白酶/大鼠Key words
Qingjin Huatan Decoction/COPD/mucus hypersecretion/MUC5AC/neutrophil elastase/rats分类
医药卫生引用本文复制引用
冯淬灵,司娜,王骏,李春盈,赵丹,冯枫,张前..清金化痰汤对慢性阻塞性肺疾病模型大鼠肺组织中性粒细胞弹性蛋白酶及黏蛋白基因表达的影响[J].中国中医药信息杂志,2015,(5):76-79,4.基金项目
北京市自然科学基金(7132115) （7132115）
