中国中医药信息杂志Issue(7)：63-66,67,5.DOI:10.3969/j.issn.1005-5304.2015.07.018
健脾活骨方不同提取部位对骨髓间充质干细胞增殖和分化的影响
Effects of Different Extracts of Jianpi Huogu Formula on Proliferation and Differentiation of Bone Marrow Mesenchymal Stem Cells
摘要
Abstract
Objective To observe effects of different extracts of Jianpi Huogu Formula (JPHGF) on proliferation and differentiation of bone marrow mesenchymal stem cell (BMSCs). Methods Whole bone marrow adherent was used to screen, culture, and isolate BMSCs. Extracts from different parts (water, chloroform, ethyl acetate and n-butanol parts) of JPHGF were administrated for a certain time. MTS was used to detent cell proliferation;ALP staining was used to detect ALP activity;ARS staining was used to detect the formation of calcium nodules;oil red O staining was used to detect fat cell formation. Results Extracts from different parts of JPHGF could promote cell proliferation of BMSCs in different levels, followed by its strength in water, chloroform, ethyl acetate, and n-butanol parts;ALP staining results showed that the intensity of ALP expression of the order is water, acetic acid ethyl, chloroform and n-butanol parts;in promoting the formation of calcium nodules, ARS staining results showed that its intensity were water, chloroform, ethyl acetate, and n-butanol parts;oil red O staining results showed that inhibition intensity of fat cells interaction strength was formed from ethyl acetate, water, chloroform to n-butanol parts. Conclusion Extracts from different parts of JPHGF have different effects on BMSCs proliferation and differentiation. Water extraction has the strongest osteogenic differentiation and proliferation, and ethyl acetate has the best effect on the inhibition of cell formation.关键词
健脾活骨方/骨髓间充质干细胞/细胞增殖/大鼠Key words
Jianpi Huogu Formula/BMSCs/cell proliferation/rats分类
医药卫生引用本文复制引用
李晓敏,孔祥英,张村,万红叶,朱嘉,陈卫衡,林娜..健脾活骨方不同提取部位对骨髓间充质干细胞增殖和分化的影响[J].中国中医药信息杂志,2015,(7):63-66,67,5.基金项目
国家自然科学基金面上项目(81173417) （81173417）
