中国中医药信息杂志Issue(7)：67-70,4.DOI:10.3969/j.issn.1005-5304.2015.07.019
化浊颗粒对2型糖尿病大鼠磷脂酰肌醇3激酶和葡萄糖转运蛋白4基因表达的影响
Effects of Huazhuo Granule on Expressions of PI-3K and GLUT4 mRNA in Rats with Type 2 Diabetes
摘要
Abstract
Objective To observe the effects of Huazhuo Granule on expression of phosphatidylinositol-3-kinase (PI-3K) and glucose transporter 4 (GLUT4) in skeletal muscle type 2 diabetes (T2DM) rats;To discuss its effects on activating signal path of PI-3K and GLUT4. Methods High-fat diet combined with low-dose streptozotocin intraperitoneal injection was used to establish T2DM rat models. The rats were randomly divided into blank group, Huazhuo Granule group, rosiglitazone group, and model group. Each treatment group was given relevant medicine for gavage. Model group and blank control group were given normal saline for gavage. The general state, weight, and food ration of rats were observed. After ten-week medicine intervention, 10%chloral hydrate was used for anesthesia to detect the levels of FBG, OGTT (2 h PG), and FINS. RT-PCR was used to detect the expressions of PI-3K and GLUT4 mRNA of rat skeletal muscle in all groups. Results Compared with model group, the levels of FBG, 2 h PG, and FINS decreased (P<0.05);the expressions of PI-3K and GLUT4 mRNA of rat skeletal muscle increased significantly (P<0.05). Conclusion Huazhuo Granule has the effects on increasing insulin sensitivity, which is related to activating insulin signal path of PI-3K and GLUT4 of skeletal muscle tissues.关键词
化浊颗粒/2型糖尿病/胰岛素抵抗/磷脂酰肌醇3激酶/葡萄糖转运蛋白4/大鼠Key words
Huazhuo Granule/type 2 diabetes/insulin resistance/PI-3K/GLUT4/rats分类
医药卫生引用本文复制引用
康学东,李菲,杨维杰,余臣祖..化浊颗粒对2型糖尿病大鼠磷脂酰肌醇3激酶和葡萄糖转运蛋白4基因表达的影响[J].中国中医药信息杂志,2015,(7):67-70,4.基金项目
甘肃省教育厅研究生导师科研项目计划(1206-06) （1206-06）
