中国中医药信息杂志Issue(7)：86-88,3.DOI:10.3969/j.issn.1005-5304.2015.07.024
不同地区板蓝根蛋白SDS-PAGE分析
Analysis on Isatidis Radix Protein in Different Areas by SDS-PAGE
杨欣 1王秋红 1王悦 1郭慧 1邢娜 1匡海学1
作者信息
- 1. 教育部北药基础与应用研究重点实验室，黑龙江省中药及天然药物药效物质基础研究重点实验室，黑龙江中医药大学，黑龙江 哈尔滨 150040
- 折叠
摘要
Abstract
Objective To identify Isatidis Radix in different areas by SDS-PAGE;To provide the basis for the identification and quality evaluation of Isatidis Radix. Methods By using SDS-PAGE technology, the protein profiles of Isatidis Radix in Heilongjiang and Hebei regions were established. Results Isatidis Radix protein in Heilongjiang contained about 13 protein subunits, and the major subunits were 10.5 kD, 23.0 kD, 24.9 kD, and 44.1 kD. Isatidis Radix protein in Hebei contained about 12 subunits, and the major subunits were 55.6 kD, 45.9 kD, 34.3 kD, 18.9 kD, 12.4 kD, and 10.5 kD. Conclusion SDS-PAGE technology can be used as one of the references to identify Isatidis Radix in different areas.关键词
板蓝根/鉴别/十二烷基磺酸钠-聚丙烯酰胺凝胶电泳Key words
Isatidis Radix/identify/SDS-PAGE分类
医药卫生引用本文复制引用
杨欣,王秋红,王悦,郭慧,邢娜,匡海学..不同地区板蓝根蛋白SDS-PAGE分析[J].中国中医药信息杂志,2015,(7):86-88,3.
