中国中医药信息杂志Issue(7)：96-99,4.DOI:10.3969/j.issn.1005-5304.2015.07.027
HPLC-DAD同时测定黄芪生脉饮中4种成分含量
Simultaneous Determination of Four Components in Huangqi Shengmai Decoction by HPLC-DAD
陆铖 1周盛会 2李炜 1郑修丽 2高庆剑 3汤浩3
作者信息
- 1. 宁夏医科大学药学院，宁夏 银川 750000
- 2. 甘肃省人民医院，甘肃 兰州 730000
- 3. 甘肃省人民医院，甘肃 兰州 730000
- 折叠
摘要
Abstract
Objective To establish a method for simultaneous determination of contents of schisandrin B, schisantherin A, lobetyolin and ruscogenin in Huangqi Shengmai Decoction with HPLC method. Methods The chromatographic column was an Agilent Eclipse C18 column (4.6 mm × 250 mm, 5 μm) that was maintained at a constant temperature of 30 ℃. The mobile phase was 0.1% acetic acid solution-acetonitrile, with gradient elution. The flow rate was 1.0 mL/min. The detection was at wavelength of 280 nm. Results The standard curves of schisandrin B, schisantherin A, lobetyolin and ruscogenin had good linearity in the ranges of 0.862 5-21.562 5μg (r 2=0.999 1), 0.737 5-18.437 5μg (r 2=0.999 4), 0.095 2-2.380 0 μg (r 2=0.999 6), and 0.810 0-20.250 0 μg (r 2=0.999 0), respectively. The average recoveries of the four components were 98.06%(RSD=1.64%), 99.61%(RSD=2.72%), 97.98%(RSD=1.45%), and 99.30%(RSD=1.25%), respectively. Conclusion This method is accurate, rapid and specific, achieving the stable results with high reproducibility.关键词
黄芪生脉饮/五味子乙素/五味子酯甲/党参炔苷/鲁斯可皂苷元/高效液相色谱法Key words
Huangqi Shengmai Decoction/schisandrin B/schisantherin A/lobetyolin/ruscogenin/HPLC分类
医药卫生引用本文复制引用
陆铖,周盛会,李炜,郑修丽,高庆剑,汤浩..HPLC-DAD同时测定黄芪生脉饮中4种成分含量[J].中国中医药信息杂志,2015,(7):96-99,4.
