中国中医药信息杂志2015，Vol.22Issue(8)：50-54,5.DOI:10.3969/j.issn.1005-5304.2015.08.014
糖网明目颗粒提取物对缺氧/高糖状态下血管内皮细胞肿瘤坏死因子-α、白细胞介素-1α表达的影响
Effects ofTangwang Mingmu Granules on Expressions of TNF-α and IL-1α in Vascular Endothelial Cellsunder Hypoxia/High Glucose
摘要
Abstract
ObjectiveTo observe the effects ofTangwang Mingmu Granules on inflammation- associated cytokines in vascular endothelial cells EA.hy926 under hypoxic/high glucose. MethodsCoCl2 and high glucose were used to make the hypoxic and high glucose model in human umbilical vein endothelial cell line EA.hy926. EA.hy926 cell proliferation was measured by MTT method. Gene and protein expression levels of inflammatory factors were detected by semi-quantitative RT-PCR and ELISA assay.ResultsBoth hypoxia and high glucose promoted EA.hy926 cell proliferation. The gene and protein expressions of TNF-α and IL-1α were significantly up-regulated under the same condition (P<0.05). The cell proliferation, TNF-α, IL-1α mRNA and protein expression levels were significantly reduced by treatment of Tangwang Mingmu Granules (P<0.05).Conclusion The preventive and therapeutic effect ofTangwang Mingmu Granules on diabetic retinopathy might be related to down-regulate the expression of inflammation-associated cytokines of mRNA and protein, such as TNF-α and IL-1α.关键词
糖网明目颗粒提取物/糖尿病视网膜病变/肿瘤坏死因子-α/白细胞介素-1α/人脐静脉内皮细胞Key words
Tangwang Mingmu Granules/diabetic retinopathy/tumor necrosis factor-α/interleukin-1α/human umbilical vein endothelial cell分类
医药卫生引用本文复制引用
蒿长英,陈明霞,郭平,马文斌,吕海波,杜佩佩,甄林娜,刘晔,连增林..糖网明目颗粒提取物对缺氧/高糖状态下血管内皮细胞肿瘤坏死因子-α、白细胞介素-1α表达的影响[J].中国中医药信息杂志,2015,22(8):50-54,5.基金项目
北京市科技计划"十病十药"中药专项(Z121102001112007) （Z121102001112007）
