中华耳科学杂志Issue(3)：481-484,4.DOI:10.3969/j.issn.1672-2922.2014.03.033
经大鼠圆窗膜bFGF基因转导防治爆震性聋的实验研究
bFGF Gene Transfer into Cochlea in Rats for Protection and Treatment in Blast Deafness
摘要
Abstract
Objective To study effects of the transduction efficiency of cationic liposome mediated EGFP-bFGF gene into the cochlea through an intact round window membrane (RWM) in rats with noise-induced hearing loss, and to assess its influence on hearing. Methods Thirty SD adult rats were divided into four groups:(1) noise exposure group:exposure to 155 dB SPL im-pulse noise 20 times only (n=6);(2) EGFP control group:cationic liposome-pEGFP complex administered immediately after im-pulse noise exposure (n=8);(3) bFGF rescue group:cationic liposome-pEGFP-bFGF complex administered immediately after impulse noise (n=8);(4) bFGF protection group:cationic liposome-pEGFP-bFGF complex administered 3 days before impulse noise (n=8). ABR thresholds were measured in all the animals before and after blast on day 1, day 3, day 7 and day 14, respec-tively. On postoperative day 14, all the animals were sacrificed, bilateral cochleae were harvested and surface preparations of basilar membrane were studied. The expression of bFGF in hair cells was detected by immunofluorescent staining. Results At day 1 after blast, the ABR threshold of the bFGF protection group was lower than the noise group and EGFP control group (P<0.05). At day 3, day 7 and day 14 after blast, the ABR threshold of the bFGF protection group and bFGF rescue group showed significant change compared with the two control groups (P<0.05). On postoperative day 14, fluorescent microscopy showed green fluorescence in the cochlea on operated side in the prevention and rescue groups. Conclusion Cationic liposome mediated bFGF gene transferred into the cochlea through the RWM can be expressed, and can partially facilitate recovery of hear-ing loss caused by impulse noise.关键词
碱性成纤维细胞生长因子/基因转导/圆窗膜/阳离子脂质体/大鼠/爆震性聋Key words
Basic fibroblast growth factor/Gene transfer/Round window membrane/Cationic liposome/Rat/Ex-plosive deafness分类
医药卫生引用本文复制引用
程晓华,郭维,翟所强,丁吉江,黄丽辉..经大鼠圆窗膜bFGF基因转导防治爆震性聋的实验研究[J].中华耳科学杂志,2014,(3):481-484,4.基金项目
卫生公益性行业科研专项基金（201202005）、首都临床特色应用研究专项基金（Z131107002213123）联合资助 （）
