中华耳科学杂志Issue(3)：503-506,4.DOI:10.3969/j.issn.1672-2922.2014.03.038
低频强噪声对大鼠听力的影响及内耳Caspase-3的表达
Effects of High Level Low Frequency Noise on Hearing and Expression of Caspase-3 in Rats
尹志利 1张建中 2吴继华 2曾新吾 3龚昌超 3田章福 3柳珑 3吴玮 1王刚 4王鸿南 4韩浩伦 4李保卫 4周丽斌 4余萌 4屈昌北4
作者信息
- 1. 安徽医科大学解放军三零六临床学院耳鼻咽喉头颈外科 北京 100101
- 2. 解放军第306医院病理科 北京 100101
- 3. 国防科技大学光电科学与工程学院 长沙 410073
- 4. 解放军第306医院耳鼻咽喉头颈外科 北京 100101
- 折叠
摘要
Abstract
Objective To study the effect of high intensity low frequency noise on hearing and apoptosis of inner ear cells. Methods Rats (n=52 ) were randomly divided into a control group (Pr) (n=13), and three noise exposure groups (n=39) that were examined immediately (E0 group, n=13), three days (E3 group, n=13) or 7 days (E7 group, n=13) following exposure to a 150 dB SPL steady noise centered around 500 Hz for 10 minutes, respectively, The auditory brainstem response was record-ed, followed by cochlear morphology examination after the animals were sacrificed and cochlear paraffin sections were prepared and stained using immunohistochemical techniques. Results Immediately after exposure to the 150 dB SPL noise, ABR thresholds were significantly elevated. Moderate recovery was seen at 3 days after exposure, with little further improvement at 7 days after exposure. ABR thresholds at all time points following exposure were higher than before exposure (P<0.01) ABR thresholds showed significant differences between the E0 group and E3 and E7 groups (P<0.05), but not between the E3 and E7 groups (P>0.05). Immunohistochemistry results showed various levels of caspase-3 expression in the Organ of Corti, spiral gan-glion cells, stria vascularis and spiral ligaments in the low-frequency sound exposure groups but not in the control group. The average optical density was different between the control and exposure groups, among the E0, E3 and E7 groups (P<0.01) and between the E3 and E7 groups (P<0.01). There was no significant caspase-3 expression in the control group. Conclusion High-intensity low-frequency noise can cause significant hearing damage, which can later partially recover. Caspase-3-medi-ated inner ear apoptosis may play an important role in the pathogenesis of noise-induced hearing loss.关键词
低频噪声/耳蜗/大鼠/凋亡/Caspase-3Key words
Low frequency noise/Cochlea/Rat/Apoptosis/Caspase-3分类
医药卫生引用本文复制引用
尹志利,张建中,吴继华,曾新吾,龚昌超,田章福,柳珑,吴玮,王刚,王鸿南,韩浩伦,李保卫,周丽斌,余萌,屈昌北..低频强噪声对大鼠听力的影响及内耳Caspase-3的表达[J].中华耳科学杂志,2014,(3):503-506,4.
