中华耳科学杂志Issue(3)：527-529,3.DOI:10.3969/j.issn.1672-2922.2014.03.045
鸡胚Bmp4和Cath1原位杂交RNA探针的制备
Preparation of An RNA Probe for In Situ Hybridization of Chicken Bmp4 and Cath1
摘要
Abstract
Objective To report a method for preparation of RNA probes for detection of Chicken Bmp4 and Cath1 in si-tu hybridization. Method Plasmids carrying specific sequences of Bmp4 and Cath1 genes were amplified using the Plasmid Mini Kit, linearized by restriction enzymes, extracted and purified by phenol-chloroform, and then synthesized to form anti-sense probes with T3 RNA polymerase in vitro. Quality of the prepared probes was tested by agarose gel electrophoresis and in situ hybridization in chicken. Results Well purified Bmp4 and Cath1 RNA probes with satisfactory results in in situ hybrid-ization were confirmed. Conclusions The reported method provides an excellent way to prepare RNA probes for chicken Bmp4 and Cath1 for in situ hybridization, which is economic, effective, with great results and excellent quality.关键词
探针制备/原位杂交/Bmp4/Cath1/鸡胚Key words
Probe preparation/In situ hybridization/Bmp4/Cath1/Chicken分类
医药卫生引用本文复制引用
汤丽川,张悦,邹艺辉..鸡胚Bmp4和Cath1原位杂交RNA探针的制备[J].中华耳科学杂志,2014,(3):527-529,3.基金项目
本研究由国家自然基金专项基金项目（31140047）（31340055），解放军总医院专利转化基金项目（42411D19）以及解放军总医院苗圃基金项目（07MP24）联合资助。 （）
