中华耳科学杂志Issue(4)：643-646,4.DOI:10.3969/j.issn.1672-2922.2014.04.033
突触内吞关键蛋白Dynamin在小鼠耳蜗内毛细胞中的表达研究
Study on the Expression of Synaptic Endocytosis Key Protein Dynamin in Mice Cochlear Inner Hair Cells
摘要
Abstract
Objective To study expression and distribution characteristics of each subtype of Dynamin in mice inner hair cell (IHC) and its possible roles in the physiological function of inner hair cells as well as laying the groundwork for study⁃ing its relationship with hearing. Methods Expression and distribution of each subtype of Dynamin in cochlear basement mem⁃brane of adult mice were distinguished by immunefluorescent, and examined by laser confocal microscope. Results Various subtypes of Dynamin were detected in the inner hair cell of mice. Of which, Dynamin-1 was seen in the entire inner hair cell, Dnamin-2 in the cytoplasm, and Dynamin-3 highly expressed under the nucleus of inner hair cells close to the spiral ganglion. Conclusion Clathrin mediated endocytosis is a major pathway of vesicle recycling, in which, Dynamin plays an important role. Various subtypes of Dynamin are expressed in the cochlear hair cells, indicating that Dynamin may have an important physio⁃logical function in inner hair cells.关键词
耳蜗内毛细胞/内吞/免疫荧光/DynaminKey words
Inner hair cell/Endocytosis/Immunofluorescence/Dynamin分类
医药卫生引用本文复制引用
蔡婷,袁伟..突触内吞关键蛋白Dynamin在小鼠耳蜗内毛细胞中的表达研究[J].中华耳科学杂志,2014,(4):643-646,4.基金项目
国家自然科学基金项目（30973301,81271080,81470694）；重庆市自然基金资助项目（NO:CSTC2009BB5170）第三军医大学基金:2010XLC06 （）
