中华耳科学杂志Issue(1)：1-6,6.DOI:10.3969/j.issn.1672-2922.2015.01.001
实验动物标准颞骨切片规范
Standardization of Temporal Bone Sections in Experimental Animals
丁大连 1于进涛 2李鹏 3高可雷 4蒋海燕 1张文娟 2孙虹 5殷善开 3Richard Salvi1
作者信息
- 1. 中南大学湘雅医院耳鼻咽喉头颈外科 湖南 410018
- 2. Center for Hearing and Deafness，Department of Communicative Disorders and Sciences，University of New York at Buffalo Buffalo NY14214
- 3. 中山大学附属第三医院耳鼻咽喉头颈外科 广州 510630
- 4. 上海交通大学附属第六人民医院耳鼻咽喉头颈外科 上海 200233
- 5. Center for Hearing and Deafness，Department of Communicative Disorders and Sciences，University of New York at Buffalo Buffalo NY14214
- 折叠
摘要
Abstract
Preparation of the temporal bone for light microscopy is an important step in histological studies of the inner ear. Due to the complexity of labyrinthine three-dimensional structures in the inner ear, it is difficult to measure or compare structures of interest without a commonly accepted standardized measure of temporal bone sections. Therefore, standardization of temporal bone sections is very important for histological assessment of sensory hair cells and peripheral ganglion neurons in the cochlear and vestibular systems. The standardized temporal bone sectioning is oriented to achieve a horizontal plane of sec⁃tioning. Horizontal sections are collected from the epitympanum to the hypotympanum including layers in the order of the crista ampullaris of the superior and lateral semicircular canals, macula utriculi and macula sacculi, superior vestibular ganglion neu⁃rons, macula of saccule and inferior vestibular ganglion neurons, cochlear modiolus, endolymphatic duct and endolymphatic sac, and finally the crista ampullaris of posterior semicircular canal. Moreover, the technical details of preparing for temporal bone sectioning including fixation, decalcification, whole temporal bone staining, embedding penetration, and embedding orien⁃tation are also discussed.关键词
实验动物标准/颞骨切片Key words
Experimental animal/Temporal section分类
医药卫生引用本文复制引用
丁大连,于进涛,李鹏,高可雷,蒋海燕,张文娟,孙虹,殷善开,Richard Salvi..实验动物标准颞骨切片规范[J].中华耳科学杂志,2015,(1):1-6,6.
