中华耳科学杂志Issue(1)：87-91,5.DOI:10.3969/j.issn.1672-2922.2015.01.016
水杨酸钠可逆性抑制耳蜗螺旋神经节神经元GABAa受体电流
Sodium Salicylate Reversibly Suppresses GABA-induced Currents in Spiral Ganglion Neurons in Rats
摘要
Abstract
Objective To study the effect of sodium salicylate (SS) on the function of GABAa receptors in spiral gangli⁃on neurons (SGNs). Methods GABAa receptor currents were recorded using whole-cell patch clamp techniques in isolated SGNs. The effects of SS were characterized. Results The currents evoked by GABA in SGNs were blocked by 10μM bicucul⁃line. No detectable currents were recorded upon using SS at 0.5 mM, 0.1 mM or 5 mM. Various degrees of reversible suppres⁃sions of GABA-induced currents were observed by administrating GABA (100μM or 500μM) combined with SS. Conclu⁃sion GABAa receptors currents can be recorded in rat cochlea SGNs. SS reversibly attenuates GABAa receptors response in SGNs. The effects are dose-dependent with both GABA and SS.关键词
水杨酸钠/GABAa受体/SGN/膜片钳技术Key words
Sodium salicylate/GABAa receptor/Spiral ganglion neurons/Whole-cell patch-clamp分类
医药卫生引用本文复制引用
蔡正,姚晨,陈慧英,黄志辉,覃继新,苏纪平..水杨酸钠可逆性抑制耳蜗螺旋神经节神经元GABAa受体电流[J].中华耳科学杂志,2015,(1):87-91,5.基金项目
国家自然科学基金项目(NO81360157,81060082)；广西自然科学基金 （NO81360157,81060082）
