中华耳科学杂志Issue(1)：151-155,5.DOI:10.3969/j.issn.1672-2922.2015.01.029
茵陈水提物对胆红素诱导神经母瘤细胞凋亡实验研究
Protective Effects by Water Extracts of Herba Artemisiae Capillariae Against Bilirubin Induced Neuroblastoma Apoptosis(SH-SY5Y)
摘要
Abstract
Objective To investigate the mechanism of protection by capillary artemisia aqueous extract (Yincheng ex⁃tract, YCE) against bilirubin induced neuroblastoma apoptosis. Methods MTT was used to assess SH-S5Y5 cells prolifera⁃tion. Western blotting was employed to examine NF-KB protein expression in the nucleus and cytoplasm. Changes of JC-1 probe labeled mitochondrial potential were examined by immunofluorescence microscopy. Annexin-FITC/PI was used to detect cell apoptosis with flow cytometry. IL-1β and TNF- alpha changes were tested using quantitative fluorescence PCR. Results YCE improved concentration and time dependent inhibition of bilirubin’s effects on cell proliferation (p<0.05). YCE inhibited NF-KB nuclear transfer that was promoted by bilirubin. On immunofluorescence microscopy, mitochondrial mem⁃brane potentials decreased significantly in the bilirubin treated group compared with the control group (p<0.05), consis⁃tent with early apoptosis, which was seen reversed by YCE treatment (p<0.05). Flow cytometry annexin-FITC/PI results showed that bilirubin induced apoptosis was countered by YCE. Fluorescence quantitative PCR showed that the expression of inflammation-related proteins IL-1 beta and TNF- alpha (regulated by the transcription factor NF-KB) was decreased by YCE (p<0.05). Conclusion While bilirubin can induce apoptosis of SH-S5Y5 cells, YCE can effectively reduce NF-KB acti⁃vation and mitochondrial membrane potential changes, and thus inhibit apoptosis induced by bilirubin at multiple target points.关键词
茵陈水提物/胆红素/神经母瘤细胞(SH-SY5Y)/凋亡Key words
Yincheng extract/YCE/Bilirubin/Neuroblastoma/Aapoptosis分类
医药卫生引用本文复制引用
张奕,梁勇,李鹏,符秋养,刘钊,李琦,张威,杨衬..茵陈水提物对胆红素诱导神经母瘤细胞凋亡实验研究[J].中华耳科学杂志,2015,(1):151-155,5.基金项目
国家自然科学基金面上项目NSFC2015 （）
