中华肝脏外科手术学电子杂志Issue(1)：53-56,4.DOI:10.3877/cma.j.issn.2095-3232.2015.01.015
siRNA沉默P2Y12受体基因对肝细胞癌血管生成能力的影响
Impact of purinergic receptor P2Y12 gene inhibited by siRNA on the angiogenesis of hepatocellular carcinoma
摘要
Abstract
Objective To investigate the impact of purinergic receptors P2Y12 gene inhibited by small interference RNA (siRNA) on the angiogenesis of hepatocellular carcinoma (HCC). Methods Human HCC cell line MHCC97H was infected using P2Y12-siRNA lentiviral expression vector and empty negative control vector to establish siRNA group and control group. The expression of P2Y12 protein in two groups was detected by Western blot assay. angiogenesis assay was used to deifne the angiogenesis potential of HCC cell. The microvessel density (MVD) of nude mice subcutaneous tumors was observed. The experimental data between two groups were compared using t test. Results The average relative expression of P2Y12 protein were 0.07±0.01 and 0.26±0.02 respectively in siRNA group and control group. The expression of P2Y12 protein in siRNA group decreased significantly compared with that in control group (t=-35.64, P<0.05). No continuous closed small tubular structure was observed in the human umbilical vein endothelial cell (HUVEC) stimulated by the supernatant of siRNA group, while large number of closed tubules was observed in the HUVEC stimulated by the supernatant of control group. A few microvessels of nude mice subcutaneous tumors were observed in siRNA group. The MVD was 5±1, which was signiifcantly lower than that in control group (23±6) (t=-17.01, P<0.05). Conclusion Purinergic receptors P2Y12 gene inhibited by siRNA can suppress the angiogenesis of HCC.关键词
受体,嘌呤能P2Y12/肝肿瘤,实验性/新生血管化,病理性Key words
Receptors, purinergic P2Y12/Liver neoplasms, experimental/Neovascularization, pathologic引用本文复制引用
邹德志,郭明明,刘江辉,蔡锐彬,徐丹苹..siRNA沉默P2Y12受体基因对肝细胞癌血管生成能力的影响[J].中华肝脏外科手术学电子杂志,2015,(1):53-56,4.基金项目
国家自然科学基金(81403341) （81403341）
