中华脑科疾病与康复杂志（电子版）Issue(5)：9-14,6.DOI:10.3877/cma.j.issn.2095-123X.2014.05.003
脑胶质瘤 MGMT 基因表达及启动子甲基化与患者临床预后关系的观察
Relationship between MGMT gene expression and promoter methylation and the clinical prognosis of patients with glioma
摘要
Abstract
Objective To investigate the influence of O 6-methylguanine-DNA methyltransferase ( MGMT) gene promoter methylation and expression in malignant glioma tissues and the clinical prognosis observation of malignant glioma patients .Methods A total of 78 postoperative patients who agreed to glioma individualized comprehensive treatment with complete clinical data were collected ,admitted to Department of Neurosurgery ,People′s Hospital of Zhengzhou University from April 2007 to April 2009 , all patients were grouped by detecting the glioma MGMT gene promoter methylation and protein expression .All patients received radiotherapy combined with chemotherapy treatment postoperation .The efficacy of two groups by observing short-term curative efficacy , survival time and the safety through the long-term follow-up were compared.The two groups of mean differences were compared by independent t-test, chi-square test was applied to count data R ×C table,and Kaplan-Meier method was used to draw survival curves ,survival curve was analyzed by Log-rank test .Results The status of MGMT gene promoter methylation demonstrate a negative correlation with MGMT protein expression ( r=-0.514 ,P<0.05 ) .The short-term curative efficacy in MGMT gene promoter methylation group was significantly superior to MGMT gene promoter unmethylation group(χ2 =47.890 ,P=0.000 ) ,and the short-term curative efficacy of low MGMT protein expression group was significantly superior to the high expression group (χ2 =30.032 ,P=0.000 ) .The survival time in MGMT gene promoter methylation group was significantly superior to the MGMT gene promoter unmethylation group (χ2 =21.405 , P <0.05 ) .And the survival efficacy time of low MGMT protein expression group was significantly superior to the high expression group (χ2 =18.643 , P<0.05 ) .The objective curative rate in MGMT gene promoter methylation group 81.0%( 34/42 ) was superior to the low MGMT protein expression group 74.4%( 29/39 ) .No adverse reaction was found in the whole treatment .Conclusion MGMT gene promoter methylation and protein expression in malignant glioma is closely related to the prognosis of patients which in treatment of application of nimustine +temozolomide rendezvous chemotherapy and conformal radiotherapy ,and MGMT gene promoter methylation is in good agreement with judging the prognosis of patients with malignant glioma , which would provide us references for making effective and individualized clinical treatment plan .关键词
神经胶质瘤/基因表达/甲基化/预后/O6-甲基鸟嘌呤-DNA甲基转移酶Key words
Glioma/Gene expression/Methylation/Prognosis/O6-methylguanine-DNA methyltransferase引用本文复制引用
袁强,步星耀,闫兆月,周志龙,孙彦熙,周伟,马春晓,屈鸣麒..脑胶质瘤 MGMT 基因表达及启动子甲基化与患者临床预后关系的观察[J].中华脑科疾病与康复杂志（电子版）,2014,(5):9-14,6.基金项目
河南省杰出人才计划项目 （）
