中华脑科疾病与康复杂志（电子版）Issue(3)：168-172,5.DOI:10.3877/cma.j.issn.2095-123X.2015.03.006
三七总皂苷对缺血再灌注大鼠神经功能及海马区葡萄糖转运蛋白-1表达的影响
Effect of panax notoginseng saponins on focal cerebral ischemia reperfusion and expression of glucose ;transporter-1 in rats
王欣 1刘志辉 2杨同 1孙文卿 1徐萍萍1
作者信息
- 1. 261042 山东省，潍坊医学院临床学院神经病学教研室1
- 2. 261042 山东省，潍坊医学院附属医院神经内科2
- 折叠
摘要
Abstract
Objective To investigate the effects of panax notoginseng saponins on neurological function of rats with ischemia reperfusion and on expression of hippocampus ′s glucose transporter 1 ( GLUT-1).Methods Sixty-eight healthy adult male wistar rats were randomly divided into sham-operate group (A),saline control group(B)and PNS therapy group of small-dose group(C1 =50 mg/kg),dose group(C2 =100 mg/kg),high-dose group(C3 =150 mg/kg),the C1,C2,C3 and B group were redivised into ischemia-reperfusion 0 h,2 h,4 h and 6 h subgroups according to the intervention time points ( n=4 in each group ) . By using the suture method to complete the rat middle cerebral artery occlusion model preparation .The neurological deficit in rats was evaluated by using behavioral score .The corresponding doses of PNS or an equal volume of saline were injected intraperitoneally administered at ischemia for 2 h and reperfusion for 0 h,2 h,4 h and 6 h in the PNS groups and the saline control group .Immunohistochemistry assay was used to detect the expressions of GLUT-1 in the hippocampus of ischemic sides at ischemia-reperfusion. Results Behavioral score:the neurological score of group A was 0.The nerve function of group C defect were significantly less than the B group ( P<0.01 ) .The group C 3 was the least among the three groups C 1 , C2 and C3 .The results of immunohistochemistry assay ,the number of GLUT-1 positive cells of group A was significantly less than group B and C ( all P<0.01 ) .The number of GLUT-1 positive cells in brain tissue of rats in the same intervention time point of the group C were significantly more than group B ( all P<0.01 ) , and group C3 was the most.Among different therapy time groups ,the expression of GLUT-1 were basically the same(P>0.05).Conclusion PNS can lessen the degree of neurologic impairment in ischemia-reperfusion rats by promoting the expression of GLUT-1,and maintain the brain energy .关键词
大鼠/再灌注损伤/皂苷类/葡萄糖转运蛋白-1Key words
Rats/Reperfusion injury/Saponins/Glucose ransporter-1引用本文复制引用
王欣,刘志辉,杨同,孙文卿,徐萍萍..三七总皂苷对缺血再灌注大鼠神经功能及海马区葡萄糖转运蛋白-1表达的影响[J].中华脑科疾病与康复杂志（电子版）,2015,(3):168-172,5.
