中华神经创伤外科电子杂志Issue(2)：27-30,4.DOI:10.3877/cma.j.issn.2095-9141.2015.02.009
创伤性脑损伤后大鼠脑线粒体功能的变化
Changes of mitochondrial function in rat brain after traumatic brain injury
摘要
Abstract
Objective To set up severe rats traumatic brain injury (TBI) model through modified Feeney free fall brain injury device and investigate the pathogenesis on brain cortical tissue mitochondrial function changes in different time. Methods Thirty adult male Sprague-Dawley (SD) rats were used in the study and randomly divided into 2 groups, sham operation group (n=5) and TBI group (n=25). TBI group was divided into five subgroups at different time points after injury (12 h, 1 d, 3 d, 5 d, 7 d) and five rats in each subgroup. The damage of neuron was observed by Nepal's staining. Mitochondrial membrane potential (MMP), H +-ATPase activity and adenosine triphosphate (ATP) content were tested by multifunctional enzyme-labeled instrument to observe the changes of cerebral cortex mitochondria after TBI. Results The post-TBI Nissl staining showed that compared with the sham group, the cortical neurons reduced in staining 12 h group, 1 d group more less, and 3 d group was the least. The MMP, activity of H+-ATPase and the content of ATP all decreased after injury, and 3 d group was the least, which had a significant difference compared to the sham operation group (P<0.05). Conclusions The post-TBI mitochondrial function in cerebral cortex first decreased and then increased, which can restore some function. This has provide a certain experimental basis on the research of post-TBI brain protection mechanism, and showed an importance to effective protection on brain injury patients.关键词
线粒体/大鼠/颅脑创伤Key words
Mitochondia/Rats/Craniocerebral trauma引用本文复制引用
王娟,程岗,王亚明,方丹东,李志超,张剑宁..创伤性脑损伤后大鼠脑线粒体功能的变化[J].中华神经创伤外科电子杂志,2015,(2):27-30,4.基金项目
国家自然科学基金资助项目(81372128) （81372128）
