转化医学杂志Issue(1)：32-36,5.DOI:10.3969/j.issn.2095-3097.2015.01.010
Notch 信号通路抑制剂对原代耐药白血病干细胞生长分化的影响
Effects of Notch inhibitor γ-secretase inhibitor on the growth differentiation of primary drug-resistant leukemia stem cells
摘要
Abstract
Objective We do some research about effects of Notch inhibitor γ-secretase in-hibitor (DAPT) on the growth differentiation of leukemia stem cells (LSCs).Methods Mononuclear cells ( MNCs ) separated from bone marrow from 9 pro-induction chemotherapy relapsed patients were cultrure in wells with DAPT .We evaluated the cells survival rate by cell proliferation inhibition experiment ,tested colony forming ability and the self-renewal capacity by stem cell colon cultivation , and observed cells morphology .We evaluated the expression levels of CD 34, CD38, CD123, CD14 by flow cytometric analysis .Results The result showed that DAPT inhibited the cells proliferation and self-renewal capacity of LSCs and increased the express of differentiation marker such as CD 14. There were more destroied cells by cultured with DAPT and less S phase cell by cultured with DAPT, more cells were division arrested in G1/G0 phase.Conclusion DAPT have inhibitory ac-tion to LSCs proliferation and self-renewal capacity .DAPT can promote the LSCs different to more maturation stage .关键词
白血病干细胞/Notch信号通路/γ-分泌酶抑制剂Key words
Leukemia stem cells (LSCs)/Notch pathway/γ-secretase inhibitor分类
医药卫生引用本文复制引用
杜宏,吴南海,栾佐..Notch 信号通路抑制剂对原代耐药白血病干细胞生长分化的影响[J].转化医学杂志,2015,(1):32-36,5.
