神经药理学报Issue(4)：8-12,5.
金莲花中荭草苷和牡荆苷对D-半乳糖致衰老小鼠脑组织动态抗氧化作用研究
Study on Dynamical-antioxidation of Orientin and Vitexin in Trollius Chinensis to Brain Tissue in D-galactose-induced Aging Mice
摘要
Abstract
Objective:To study antioxidant enzyme activity and lipofuscin dynamic effects of orientin and vitexin in Trollius chinensis on aging mice induced by D-galactose(D-Gal)and find the time effect relationship of antioxidant. Methods:The aging mice model was induced by intraperitoneal injection of D-Gal,and it was successful after 8 weeks. Divided into blank group,model group,high,medium and low dose group of orientin and vitexin,Vitamin E control group. From the 9 th week,every morning each group will be given medicine. The route was oral administration,and the time was 8 weeks. Decapitated mice at the 2 th ,4 th ,6 th and 8 th week,resected brain in frozen conditions,10% tissue homogenate was made with NS solution, then got supernatant by use of centrifugation in 8 000 rpm,detected superoxide dismutase(SOD), catalase(CAT),glutathione peroxidase(GSH-PX)and lipofuscin(LPF). Results:Compared with model group,the activity of antioxidase in each therapy group increased slowly after 2 and 4 weeks,but not statistically significant,and after 6 and 8 weeks,orientin and vitexin significantly improved the activity of antioxidase,and significantly reduced the amount of LPF. Compared orientin with vitexin,the activity of antioxidase and the amount of LPF in the same dose group was no statistically significant after 2 and 4 weeks. The activity of antioxidase in each dose group of orientin was better than that of the vitexin of the same dose. They significantly reduced lipofuscin levels in the brain after 6 weeks and orientin was superior to vitexin at the same dose. The activity of antioxidase in medium and low dose group of orientin was better than the same vitexin,they significantly reduced lipofuscin levels in the brain and high dose group have no significant difference after 8 weeks.Compared with VE group,after 8 weeks,the activity of antioxidase in medium and low dose group of orientin and vitexin were lower than VE group, they have significant difference,meanwhile,high dose group have no significant difference. Conclusion:Orientin and Vitexin can apparently improve activity of antioxidation and reduce lipofuscin levels in brain. The activity of antioxidase became increased with the concentration increasing and reaction time prolonging. After 8 weeks,high dose group of orientin and vitexin have the same function with VE.关键词
荭草苷/牡荆苷/D-半乳糖/抗氧化/脑组织Key words
orientin/vitexin/D-galactose/antioxidation/brain tissue分类
医药卫生引用本文复制引用
田青青,安芳,王书华..金莲花中荭草苷和牡荆苷对D-半乳糖致衰老小鼠脑组织动态抗氧化作用研究[J].神经药理学报,2012,(4):8-12,5.基金项目
河北省张家口市科技局资助项目（No.11110015D），河北北方学院 （）
