神经药理学报Issue(1)：17-22,6.
6-羟多巴胺诱导帕金森病大鼠模型行为学评价方法的探讨
Assessment of 6-hydroxydopamine-Lesion Induced Behavioral Alteration as a Rat Model of Parkinson’s Disease
摘要
Abstract
Objective:To systematically evaluate 6-hydroxydopamine(6-OHDA)-induced behavioral alteration as a rat model of Parkinson’s disease. Methods:6-OHDA was microinjected into the left side of the substantia nigra striatum to damage the dopaminergic neurons in the SD rats. Three weeks later,intraperitoneal injection of apomorphine(APO)to observe the rotational behavior. The motoric function of animals was analyzed with rotarod test and open field test, and the rat’s muslce vibration frequency and amplitude were determined using the myoelectricity test. The severity of the behavioral alterations of the individual animals was also categorized. Results:The time of rats that remained on the rotarod was significantly reduced in model group as compared to sham group. In the open field test,the horizontal travel distance was decreased in the model group. The myoelectricity test result showed that the muscle vibration frequency and amplitude was increased in animals receiving 6-OHDA microinjection. Conclusion:These results provided behavioral evidence in future studies to evaluate and categorize Parkinson-like behaviors in rats.关键词
帕金森病/动物模型/行为学/评价方法Key words
Parkinson’s disease(PD)/animal models/behavioral science/evaluation method分类
医药卫生引用本文复制引用
杨谦谦,孙芳龄,艾厚喜,张丽,王文..6-羟多巴胺诱导帕金森病大鼠模型行为学评价方法的探讨[J].神经药理学报,2013,(1):17-22,6.基金项目
“重大新药创制”科技重大专项项目（No.2012ZX09102-201-016），国家自然科学基金项目（No.81173575），北京市自然科学基金项目（No.7062031），北京市教委科技创新平台项目 （）
