神经药理学报Issue(3)：8-14,7.
应用均匀设计-高通量筛选技术评价丹参有效单体成分抗氧化及海马神经细胞保护作用的最佳配伍
Screening the Optimal Combination Ratio of Active Radix Salviae Miltiorrhizae Ingredients for Their Antioxidant and Hippocampal Neurons Protection Effects Using Uniform Design-High Throughput Screening Technology
摘要
Abstract
Objective:To implement uniform design-high throughput screening(UD-HTS) technology for screening the antioxidant and hippocampal neurons protection activities of radix salviae miltiorrhizae(RSM)ingredients using multifactor and multilevel combination design. Methods: Bioactive ingredients of RSM(7 liposoluble and 7 water-soluble)were combined with different ratios through uniform design. The bioactivity of the combination samples were assessed by anti-DPPH oxidation activity method(screening)and cytological experiment (rescreening). Results: Two optimal combination samples of the antioxidant and hippocampal neurons protection, A13 and PA, were identified. Conclusion:UD-HTS technology is suitable for multifactor and multilevel screening, which can provide rapid screening of optimal combinations.关键词
丹参/均匀设计-高通量筛选/2,2-二苯基-1-苦肼基自由基/海马神经细胞Key words
radix salviae miltiorrhizae/uniform design-high throughput screening(UD-HTS)/2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl(DPPH)/hippocampal neurons分类
医药卫生引用本文复制引用
金灿,张丹参..应用均匀设计-高通量筛选技术评价丹参有效单体成分抗氧化及海马神经细胞保护作用的最佳配伍[J].神经药理学报,2013,(3):8-14,7.基金项目
国家自然科学基金项目（No.81274005），河北省自然科学基金项目 （）
