神经药理学报Issue(5)：38-42,5.
局灶性脑缺血-再灌注大鼠Wnt3a蛋白表达的变化
Temporal Changes of Wnt3a Protein Level in Rats after Focal Cerebral Ischemia Reperfusion
摘要
Abstract
[ABSTRACT]Objective: To investigate the dynamic change of Wnt3a in rats after focal cerebral ischemia reperfusion.Methods: 80 rats were induced by occlusion of middle cerebral artery with suture embolus and ischemia for 30 minutes,then randomly assigned into different groups to receive reperfusion for 3,6,12,24 h,3,7,14,28 d. Sham operation group did not receive no suture embolus. The infarct side of the cortex of rats for each time group was harvested for the assessment of protein expression of activated Wnt3a using western blot staining. Results: Compared with the sham operation group(0.66±0.04),Wnt3a was slightly increased after 3 hours of focal cerebral ischemia reperfusion,and then reached the peak at 24 hours(1.57±0.18) and maintained a higher level within 7 days,declined after 2 weeks,but remained higher than the level of the sham operation group at 4 weeks(0.68±0.04).Conclusion: Wnt3a level of the cortex changed over time afterreperfusion,suggesting that this signaling pathway may be activated in the regulation of neurogenesis.关键词
脑缺血-再灌注/Wnt3a/动态变化/大鼠Key words
reperfusion of cerebral ischemia/Wnt3a/dynamic change/rat分类
医药卫生引用本文复制引用
李艳菲,王文..局灶性脑缺血-再灌注大鼠Wnt3a蛋白表达的变化[J].神经药理学报,2013,(5):38-42,5.基金项目
“重大新药创制”科技重大专项项目（No.2012ZX09102201-016），国家自然科学基金项目 （）
