针灸临床杂志Issue(7)：57-60,4.
电针预处理对慢性应激抑郁模型大鼠海马内5-HT及5-HT转运体表达的影响
Effect on the Expression of 5-HT,5-HTT in Hippocampus of Chronic Unpredictable Mild Stress-induced Depressive Rats by Electro-acupuncture Pretreatment
摘要
Abstract
Objective:To investigate the effect on the expression of 5-HT,5-HTT in the hippocampus of rats with chronic unpredictable mild stress by electro -acupuncture ( EA) treatment and EA pretreatment , to eluci-date the possible role of 5-HT,5-HTT in the development of experimental depression and the effect of EA on them.Methods:60 adult male SD rats were randomly divided into normal control group (n=12), model group ( n=12 ) , pulsed EA group ( n=12 ) , EA pretreatment group ( n=12 ) and fluoxetine group ( n=12 ) .Depres-sive model was established by lonely raising and chronic unpredictable mild stress for 21 days.Open-field test was performed to test the behavior of rats .The expressions of hippocampus 5-HT,5-HTT were detected by immunohistochemistry technique .Results:The cross and rear scores of the model group were significantly lower than those in normal control group , while pulsed EA group , EA pretreatment group and fluoxetine group could reverse it.The expressions of hippocampus 5-HT,5-HTT in model group were decreased significantly .The three kinds of treatments could improve these pathological change , and there was no statistical difference among these three treatments in the aspect of increasing 5-HT.While in the aspect of increasing 5-HTT, EA pre-treatment had the tendency of increasing 5-HTT,and the effect of EA and fluoxetine were better than the EA pretreatment .Conclusion:The levels of 5-HT,5-HTT in hippocampus of depression rats are lower than nor-mal.EA treatments may have antagonism against depression by increasing the levels of 5-HT,5-HTT in hip-pocampus ,and there is no difference between the effects of pulsed EA treatment and EA pretreatment on depres-sion .关键词
电针预处理/抑郁症/大鼠/海马/5-羟色胺/5-羟色胺转运体Key words
Electro-acupuncture pretreatment/Depression/Rats/Hippocampus/5-HT/5-HTT分类
医药卫生引用本文复制引用
李昱颉,李志刚,莫雨平,邓晓丰,纪倩,姜婧,周源,曹瑾,宋良玉,杨娟..电针预处理对慢性应激抑郁模型大鼠海马内5-HT及5-HT转运体表达的影响[J].针灸临床杂志,2014,(7):57-60,4.基金项目
国家自然基金项目，编号81072860。 （）
