针灸临床杂志Issue(12)：54-56,3.
骨髓间充质干细胞联合电针对大鼠糖尿病周围神经病神经营养因子表达的影响
Effect of Electroacupuncture Combined with Bone Marrow Mesenchymal Stem Cells on Neurotrophic Factors of Rat Models with Diabetic Peripheral Neuropathy
摘要
Abstract
Objective:To investigate the effect of electroacupuncture( EA) combined with bone marrow mesen-chymal stem cells ( BMSC) transplanted on the expression of nerve growth factor ( NGF) and neurotrophins-3 (NT-3) in the sciatic nerve of rats with diabetic peripheral neuropathy(DPN).Methods:The rat models with diabetic peripheral neuropathy were established by streptozotocin peritoneal injection method.Healthy SD rats were randomly divided into a model group,an EA group,a BMSC group and an EA+BMSC group.EA treat-ment was applied to“Ganyu”,“Piyu” ,“Shenyu” and“Zusanli” in EA group;BMSC suspension was infused into the caudal vein in BMSC group;EA+BMSC group was combined EA with BMSC.14d after modeling, the conduction velocity of sciatic nerve was measured,and the mRNA expressions of NGF and NT-3 in the sciatic nerve were detected by RT-PCR.Results:Compared with the model group, the conduction velocity of sciatic nerve was significantly increased in EA group, BMSC group and EA+BMSC group (P<0.01),and the mR-NA expressions of NGF, NT-3 in sciatic nerve were significantly increased (P<0.01).Compared with EA group and BMSC group, the conduction velocity was significantly increased in EA+BMSC group, and the ex-pressions of NGF and NT-3 were increased (P<0.05).Conclusion:The combined treatments promote con- <br> duction velocity of sciatic nerve more significantly than either of the single treatment in DPN rats , which may be related to the combined treatments which have synergistic effect on increasing the expressions of NGF and NT-3 .关键词
骨髓间充质干细胞/电针/大鼠/糖尿病周围神经病/神经生长因子/神经营养因子3Key words
Bone marrow mesenchymal stem cells/Electroacupuncture/Rats with diabetic peripheral neuropa-thy/NGF/NT-3分类
医药卫生引用本文复制引用
王晓莉,覃建洋,尹昌浩,张忠敏..骨髓间充质干细胞联合电针对大鼠糖尿病周围神经病神经营养因子表达的影响[J].针灸临床杂志,2014,(12):54-56,3.基金项目
黑龙江省卫生厅科研课题资助项目，编号2011－304。 （）
