浙江医学Issue(10)：867-870,919,5.
透明质酸支架复合小鼠3T3- L1细胞及VEGF注射充填的实验研究
The study of injection of 3T3-L1 preadipocyte and hyaluronic acid scaffold combined with VEGF
童芸 1王晓芬 1吴芳 1郭梅菊 1沈辉2
作者信息
- 1. 321000 金华市中心医院整形美容中心
- 2. 浙江大学医学院附属第一医院
- 折叠
摘要
Abstract
Objective To evaluate the effect of increasing the survival rate of mouse 3T3- L1 after transplantation with hyaluronic acid (HA) gel scaffold and vascular endothelial growth factor (VEGF). Methods After cellculture and differentiation induction in vitro, gene expression of peroxisome proliferator activated receptor γ (PPARγ) and CCAAT/enhancer- binding proteinα (C/EBPα) was tested. HA- VEGF group (3×106 induced 3T3- L1+0.6ml PBS+0.6ml HA), HA group (3×106 induced 3T3+0.6mlPBS +0.6mlHA), VEGF group (3 ×106 induced 3T3+1.2mlPBS +20ng/mlVEGF) and control group (3 ×106 induced 3T3+1.2mlPBS) were set up to transplant the components. The mice were executed after 2, 4, and 8 weeks. Morphology and pathology evaluation were performed to exam the tissue specimen. The weight measurement of the tissue specimen and the microvessel counting were also performed. Results After culture and differentiation induction in vitro, the band density of PPARγand C/EBP α were similar between groups by PCR test. The HE and Sultan III staining result indicated that VEGF combined with hyaluronic acid transplantation group had regular cellsize, more cellsurvival, less necrosis than other groups. After 2, 4 and 8 weeks, Graft weight of HA- VEGF group was significant higher than other groups. Graft weight of HA groups was significant higher than VEGF group and control group. The results had statistical difference (P<0.05). After 2, 4 and 8 weeks, the microvessel density of HA- VEGF group was significant higher than other groups. Microvessel density of VEGF groups was significant higher than HA group and control group. The results had statistical difference (P<0.05). Conclusion Intradermal injection of 3T3- L1 preadipocyte by hyaluronic acid scaffold combined with VEGF increases the rate of survival of transplanted tissue.关键词
透明质酸/小鼠/3T3-L1细胞/血管内皮细胞生长因子Key words
Hyaluronic acid/Mouse/3T3- L1 preadipocyte/VEGF引用本文复制引用
童芸,王晓芬,吴芳,郭梅菊,沈辉..透明质酸支架复合小鼠3T3- L1细胞及VEGF注射充填的实验研究[J].浙江医学,2014,(10):867-870,919,5.
