浙江医学Issue(12)：1034-1037,4.
靶向干扰CDK2AP1对乳腺癌MCF-7细胞体外迁移和侵袭的影响
CDK2AP1 knockdown inhibits migration and invasion of human breast cancer MCF-7 cell line in vitro
摘要
Abstract
Objective To investigate the role of cyclin- dependent kinase 2- associated protein 1(CDK2AP1) in migration and invasion of human breast cancer cells. Methods Recombinant lentivirus pGCLV- CDK2AP1- shRNA and negative control pGCLV- GFP were constructed and transfected to human breast cancer MCF- 7 cells. Migration and invasion ability of MCF- 7 cells were determined with xCELLigence system and Transwel chamber assay, respectively. Results Recombinant lentivirus pGCLV- CDK2AP1- shRNA and negative control pGCLV- GFP were transfected into MCF- 7 cells successful y. After transfection, expression levels of CDK2AP1 mRNA in MCF- 7 cells were reduced significantly(P<0.05). Compared to control MCF- 7/RNAi- NC cells, MCF- 7/RNAi- CDK2AP1 significantly decreased the migration and invasion ability of MCF- 7 cells. Conclusion Si-lence of CDK2AP1 significantly inhibits migration and invasion of human breast cancer MCF- 7 in vitro.关键词
乳腺癌/CDK2AP1 shRNA/细胞迁移/细胞侵袭Key words
Human breast cancer/CDK2AP1 shRNA/cellMigration/cellInvasion引用本文复制引用
魏任雄,李克强,毛联钢,李旭军,冯伟云..靶向干扰CDK2AP1对乳腺癌MCF-7细胞体外迁移和侵袭的影响[J].浙江医学,2014,(12):1034-1037,4.基金项目
宁波市自然科学基金资助项目 （）
