浙江医学Issue(13)：1133-1136,4.
TGF-β1对哮喘大鼠气道平滑肌细胞增殖的影响研究
Effect of TGF-β1 on proliferation of airway smooth muscle cells in asthma rats
摘要
Abstract
Objective To investigate the effect of TGF- β1 on the proliferation of airway smooth muscle cells (ASMCs) in asthma rats. Methods Chronic asthma model was induced in rats and airway smooth muscle cells were isolated and cultured in vitro. The cultured ASMCs were divided into normal group, asthma group, TGF- β1 group and TGF- β1+PD- 98059 group. The cellproliferation was determined with CCK- 8 method and the expression of caveolin- 1 and p- ERK1/2 protein was detected with Western blot. Results The expression of p- ERK1/2 increased significantly in TGF- β1 group compared with other 3 groups (P<0.05), while the expression in TGF- β1+PD- 98059 group was higher than that in asthma group (P<0.05). The expression of caveolin- 1 in TGF- β1 group was lower than that in the normal group and asthma group as wel as in TGF- β1+PD- 98059 group (P<0.05). Conclusion TGF- β1 can down- regulate the expression of protein caveolin- 1 to activate the ERK pathway, thereby to promote the proliferation of airway smooth muscle cells, which final y causes airway remodeling.关键词
转化生长因子- β1/哮喘/气道平滑肌细胞/微囊蛋白- 1/ERKKey words
TGF- β1/Asthma/ASMC C/aveolin- 1/ERK引用本文复制引用
吴立琴,戴元荣,李凤琴,王瑞丽,曾潍贤..TGF-β1对哮喘大鼠气道平滑肌细胞增殖的影响研究[J].浙江医学,2014,(13):1133-1136,4.基金项目
浙江省卫生厅课题 （）
