浙江医学Issue(15)：1310-1313,1320,5.
RNA干扰技术干涉后核糖体蛋白S15a在胃癌中的表达及意义
Knock-down of RPS15a by siRNA inhibits proliferation of gastric cancer BGC823 cells
摘要
Abstract
Objective To investigate the effects of ribosomal protein S15a (RPS15a) in proliferation of gastric cancer cellBGC823 by RNA interference technique. Methods BGC823 cells were transfected with random non- coding sequences (siNC group), siRPS15a- 1 (siRPS15a- 1 group) or siRPS15a- 2 (siRPS15a- 2 group), respectively. RPS15a RNA and protein expression was detected by RT- PCR and Western Blot test, respectively;and the cellproliferation was determined by flow cytometry. Re-sults The expression levels of RPS15 in SiRPS15a- 1 and siRPS15a- 2 groups were significantly lower than those in non- trans-fected BGC823 cells (WT group) and siNC group (al P<0.05); no significant differences was found between WT and siNC groups (P>0.05). cellproliferation rate were decreased significantly in SiRPS15a- 1 and siRPS15a- 2 groups, and most cells were in G0/G1 phase. Conclusion RPS15a RNA interference technique inhibits cells growth, which is associated with the knock- down of RPS15a expression in BGC823 cells.关键词
核糖体蛋白/S15a/胃癌/RNA干扰Key words
Ribosomal protein S15a(RPS15a)/Gastric cancer (GC)/RNA interference (RNAi)引用本文复制引用
刘勇攀,石定,王泽军,于香..RNA干扰技术干涉后核糖体蛋白S15a在胃癌中的表达及意义[J].浙江医学,2014,(15):1310-1313,1320,5.基金项目
杭州市卫生局计划项目 （）
