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对废弃胚胎行囊胚培养在体外受精-胚胎移植中的意义

王荣 费小阳 王丽卿 邹立波

浙江医学Issue(17):1447-1449,3.
浙江医学Issue(17):1447-1449,3.

对废弃胚胎行囊胚培养在体外受精-胚胎移植中的意义

Potential use of discarded embryos for in vitro fertilization and embryo transfer (IVF-ET)

王荣 1费小阳 1王丽卿 1邹立波1

作者信息

  • 1. 321000 金华市人民医院生殖中心
  • 折叠

摘要

Abstract

Objective To explore the potential use of discarded embryos for in vitro fertilization- embryo transfer (IVF- ET). Methods The discarded embryos from d3 were cultured into blastula by the sequential culture method. Results A total of 1962 discarded embryos from 782 patients were cultured, in which 376 blastocysts were formed with a blastocyst rate of 19.2%. The blastocyst formation rates were significantly higher from 1PN, 0PN and>6 cells embryos. The clinical pregnancy rate in women with blastosphere formed was significantly higher than those without blastosphere formed. Sixty four blastocysts were formed for 2PN,10 of which were transferred while the others were frozen. The clinical pregnancy rate of blastocyst transfer was 66.7%. Thir-teen women got pregnant in 24 frozen- thawed blastocyst transfer cycles. A total of 18 healthy babies were born from blastocyst transfer. Conclusion The discarded embryos have different development potential.They can develop into biastospheres. Blas-tocyst from appropriate discarded embryos can improve the utilization rate of embryos.

关键词

体外受精- 胚胎移植/废弃胚胎/囊胚/序贯培养

Key words

IVF- ET/Discarded embryos/Blastocyst/Sequential culture

引用本文复制引用

王荣,费小阳,王丽卿,邹立波..对废弃胚胎行囊胚培养在体外受精-胚胎移植中的意义[J].浙江医学,2014,(17):1447-1449,3.

基金项目

金华市科技局科技项目 ()

浙江医学

OACSTPCD

1006-2785

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