浙江医学Issue(18)：1524-1526,1571,4.
丹皮酚抑制鱼藤酮诱导的SH- SY5Y细胞凋亡的实验研究
Paeonol prevents SH-SY5Y cells from rotenone-induced apoptosis
摘要
Abstract
Objective To investigate the effects of paeonol on rotenone- induced apoptosis in SH- SY5Y cells. Methods SH- SY5Y cells were pretreated with paeonol and then rotenone was added in the culture medium. cellviability was detected by CCK- 8 assay. The apoptosis rate was assessed by flow cytometry. Cellular ROS production were detected by DCFH- DA. The mitochondial membrane potential (MMP) was determined by JC- 1 staining and the caspase- 3 activity was measured by cas-pase- 3 kit. Results 1μmol/L rotenone significantly decreased cellviability, enhanced the apoptotic rate and overproduced ROS, reduced MMP and increased caspase- 3 activity. Compared to rotenone treated group, 5,25μmol/L paeonol significantly ameliorated celldamage, inhibited apoptotic rate and ROS overproduction, al eviated the decrease of MMP and reduced the caspase- 3 activity. Conclusion Paeonol can prevent SH- SY5Y cells from apoptosis induced by rotenone, which may be as-sociated with its anti- oxidant ability.关键词
丹皮酚/鱼藤酮/帕金森病/凋亡Key words
Paeonol/Rotenone/Parkinson disease/Apoptosis引用本文复制引用
王浩,耿赵铭,胡智伟,呙登俊..丹皮酚抑制鱼藤酮诱导的SH- SY5Y细胞凋亡的实验研究[J].浙江医学,2014,(18):1524-1526,1571,4.基金项目
浙江省自然科学基金项目 （）
