浙江医学Issue(20)：1677-1680,4.
尿沉渣2种miRNA检测在膀胱癌诊断中的应用价值
miR-142-3p and miR-145-5p in urine sediment as potential markers for bladder cancer
摘要
Abstract
Objective To investigate the contents of miR- 142- 3p and miR- 145- 5p in urine sediment of patients with bladder cancer. Methods Morning mid- stream urine samples were col ected from 51 patients with early stage bladder cancer before transurethral resection and 38 healthy controls. RNAs were extracted from urine sediment and targeting miRNAs were quantified by stem- loop qPCR. The area under receiver operating characteristic curve (AUC- ROC) was used to assess the di-agnostic value of miRNAs for bladder cancer. Results miR- 142- 3p in urine sediment was significantly elevated in urinary sediment of bladder cancer patients (FC=3.93, P=0.0002, AUC=0.736), while the miR- 145- 5p had a trend of reduction (FC=0.59, P=0.056). The ratio of miR- 145- 5p/miR- 142- 3p was significantly lower in early bladder cancer patients than that in healthy controls (FC=0.15, P=0.00004, AUC=0.755). Conclusion MiR- 142- 3p in urine sediment is increased in patients with early bladder cancer, and the ratio of miR- 145- 5p/miR- 142- 3p may be used for early detection of bladder cancer.关键词
miRNA/膀胱癌/肿瘤标志物Key words
MiRNA/Bladder cancer/Tumor biomarker引用本文复制引用
阎家骏,徐国强,沈翀,孙泽宇,任煜,周毅,李俊龙,唐桂良,潘建刚,潘寿华..尿沉渣2种miRNA检测在膀胱癌诊断中的应用价值[J].浙江医学,2014,(20):1677-1680,4.基金项目
浙江省科技厅公益技术应用研究计划项目 （）
