浙江医学Issue(22)：1847-1850,4.
耐青霉素肺炎链球菌的蛋白质组学研究
Proteomic analysis of penicillin-resistant streptococcus pneumoniae strain
摘要
Abstract
Objective To analyze the proteomics of penicil in- resistant streptococcus pneumonia (PRSP). Methods The penicil in- resistant streptococcus pneumonia strain was induced by sub- MIC method. The protein profile of Streptococcus pneumoniae and induced PRSP were examined by two- dimensional electrophoresis;and the electrophoretic patterns were ana-lyzed by using Image Master 2D Platinum 5.0. The differential y expressed proteins were further identified by mass spectrometry. Results PRSP strains were induce successful y. Ten differential y expressed proteins were identified between Streptococcus pneumoniae and induced PRSP. The expression of ABC transporter, trigger factor, DNA Polymerase III, amino acid transport, pneumococcal surface protein A was increase in PRSP;while the expression of chaperonin GroES, lipoprotein, fructose bisphos-phate aldolase,α- enolase decreases, hypothetical protein SpneT_02001699 was decreased. Conclusion The findings of pro-teomics change in penicil in- resistant streptococcus pneumoniae may provide laboratory evidence for further study of drug re-sistance mechanisms.关键词
耐青霉素肺炎链球菌/蛋白质组学/双向电泳/质谱分析Key words
PRSP/Proteomic/2- DE/Mass spectrometry引用本文复制引用
王勇,卜劲松..耐青霉素肺炎链球菌的蛋白质组学研究[J].浙江医学,2014,(22):1847-1850,4.
