浙江医学Issue(24)：1973-1976,1989,5.
黄芪甲苷抑制糖尿病大鼠足细胞凋亡的作用和机制研究
Inhibitory effects of astragaloside IV on apoptosis of podocytes and its mechanism in diabetic rats
摘要
Abstract
Objective To investigate the effects of astragaloside IV (AS- IV) on apoptosis of podocytes and its mech-anism in diabetic rats. Methods Male SD rats were randomly divided into normal control group, diabetes group and diabetes with AS- IV treatment group with 10 in each group. Diabetes was induced by STZ intraperitoneal injection in rats. Rats in treatment group were given AS- IV 10mg/kg/d for 8 weeks. At the end of the 8th week, 24h microalbumin, body weight, blood glucose, blood glycated hemoglobin A1c, alanine aminotransferase, blood urea nitrogen and creatinine were measured;renal pathological changes were evaluated by light microscopy with PAS and Masson staining; glomerular podocyte density was examined by WT- 1 immunohistochemical staining;podocyte apoptosis was detected by TUNEL;the mRNA and protein expression of Bax and Bcl- 2, apoptosis- related proteins in rat renal cortex were determined by Western blot and real time- PCR. Results Hyper-glycemia, increased 24h microalbumin, mesangial expansion, interstitial fibrosis, podocyte loss and podocyte apoptosis, in-creased mRNA and protein expression of Bax and decreased mRNA and protein expression of Bcl- 2 were detected in diabetic rats. AS- IV treatment inhibited podocyte apoptosis, ameliorated podocyte loss and renal histopathology, decreased microalbu-min, partial y restored mRNA and protein expression of Bax and Bcl- 2. Conclusion AS- IV may inhibit podocyte apoptosis and ameliorate diabetic nephropathy by inhibiting the expression of Bax and restoring the expression of Bcl- 2 in diabetic rats.关键词
黄芪甲苷/糖尿病肾病/足细胞/凋亡Key words
Astragaloside IV/Diabetic nephropathy/Podocyte/Apoptosis引用本文复制引用
陈宜方,陈建国,王虹,郑志贵,崔晓萌,何东元..黄芪甲苷抑制糖尿病大鼠足细胞凋亡的作用和机制研究[J].浙江医学,2014,(24):1973-1976,1989,5.基金项目
浙江省自然科学基金项目 （）
