浙江医学Issue(3)：184-188,5.
内质网应激在蛛网膜下腔出血后早期脑损伤中的作用机制
Autophagy is involved in endoplasmic reticulum stress-related neuroprotection against apoptosis in rat subarachnoid hemorrhage
摘要
Abstract
Objective To investigate whether autophagy activation participates in the neuroprotective effects of endo-plasmic reticulum (ER) stress- related neuroprotection in rat subarachnoid hemorrhage. Methods The rats were treated with ER stress inducer tunicamycin (Tm) or inhibitor tauroursodeoxycholic acid (TUDCA) intraperitoneal y, then subarachnoid hemor-rhage (SAH) was induced by endovascular perforation was performed in a total of 84 rats to induce. Autophagy inhibitor 3- methyladenine (3- MA) was administrated by intracerebral ventricular infusion to determine the relationship between autophagy and ER stress. Neurological evaluation was performed at 24 h after SAH, then al animals were sacrificed and their brain samples were col ected for molecular biology and histology studies. Results ER stress level was increased in rats after 24 h of SAH. Pre-treatment with Tm significantly improved neurological deficits, reduced the expression of caspase- 3 and the number of TUNEL- positive cel s. Pretreatment with TUDCA significantly aggravated neurological deficits and cel apoptosis. Western blot analysis revealed that levels of the autophagic protein Beclin 1 and the ratio of LC3- II to LC3- I were increased in Tm group and reduced in TUDCA group. Pretreatment with 3- MA blocked the Tm- induced anti- apoptotic effects. Conclusion Autophagy ac-tivation might contribute to ER stress- related neuroprotection in early brain injury fol owing SAH in rats.关键词
内质网应激/蛛网膜下腔出血/早期脑损伤/自噬/凋亡Key words
Endoplasmic reticulum stress/Subarachnoid hemorrhage/Early brain injury/Autophagy/Apoptosis引用本文复制引用
张立新,严锋,王林,陈高..内质网应激在蛛网膜下腔出血后早期脑损伤中的作用机制[J].浙江医学,2015,(3):184-188,5.基金项目
国家自然科学青年基金项目 （）
