浙江医学Issue(12)：1037-1040,1045,5.
酪酸梭菌抗阿司匹林致小鼠胃溃疡作用的初步研究
Clostridium butyricum prevents gastric ulcer induced by aspirin in mice
摘要
Abstract
Objective To investigate the effect of Clostridium butyricum (C. butyricum) in prevention of gastric ulcer (GU) induced by aspirin in mice. Methods Thirty ICR mice were randomly divided into model group, bacteria group andomeprazole group (n=10/group). After starvation for 48h, mice were given oral y with 250mg/kg aspirin to establish GU model.Mice in bacteria group were oral y pretreated with C. butyricum 6 ×108cfu for 4d before starvation, while mice in model group and omeprazole groupwere given the samevolume of sterile culture medium.Mice in omeprazole group were intraperitoneal y injected with 13mg/kg omeprazole 30 min priorto aspirin treatment. Modeling for 4 h, gastric tissue was to harvest for measuring the activity of SOD and CAT, the content ofMDA, Bcl- 2 and Bax, HE staining and PAS staining were performed. Results In model group, the activity of SOD, CAT were significantly reduced while the content of MDA was significantly increased. The activity of SOD,CAT were in-creased while the content of MDA was reduced in bacteria group (P<0.01), there was no significant difference between bacteria and omeprazole group. The Bcl- 2 expression increased and Bax expression reduced in bacteria group. PAS stainning showed that the mucus secretion was increased in bacteria group and HE staining showed that the mucosa injury was attenuated in bacte-ria gorup. Conclusion C. butyricum can effectively protect the gastric mucosa against damages caused by aspirin in mice.关键词
酪酸梭菌/阿司匹林/胃溃疡/氧化应激Key words
Clostridium butyricum/Aspirin/Gastric ulcer/Oxidative stress引用本文复制引用
沈淑蓉,朱静,张浩,曾静静,付瑶阳,王雅琪,王方岩..酪酸梭菌抗阿司匹林致小鼠胃溃疡作用的初步研究[J].浙江医学,2015,(12):1037-1040,1045,5.基金项目
温州市科技局项目（Y20130215，Y20110136）；浙江省新苗计划项目 （）
