肿瘤药学Issue(4)：263-266,4.DOI:10.3969/j.issn.2095-1264.2014.053
IL-37对宫颈癌HeLa细胞增殖和凋亡的影响
Effects of IL-37 Gene on Growth and Apoptosis of HeLa Cells
蒋娟 1邹勇德 2蒋福平 3陈力3
作者信息
- 1. 永州市冷水滩区妇幼保健院妇产科，湖南 永州，425000
- 2. 永州市中心医院，湖南 永州，425000
- 3. 永州市第三人民医院，湖南永州，425000
- 折叠
摘要
Abstract
Objective To investigate the effect of inflammation inhibitor IL-37 on the growth and apoptosis of cervical cancer cell line HeLa. Methods The shuttle vector pAd track CMV that carried the objective gene of IL-37 was transfected into HeLa cells. Effects of IL-37 on growth and apoptosis of the transfected cells were evaluated by Methyl thiazolyl tetrazo-lium (MTT) rapid photocolorimetric assay and flow cytometry (FCM). At the same time, pAd track CMV was transfected into HeLa cells as empty vector control while wild type HeLa cells as negative control. Results Fluorescence microscopy showed that IL-37 plasmid was successfully transfected into HeLa cells. MTT assay showed that 12 h~96 h after transfection, the OD of IL-37 transfected HeLa cells was significantly lower than the empty vector control group (P<0.01). But the OD showed no significant difference between the empty vector control group and negative control group had no significant dif-ference;FCM results showed that the early apoptosis rate of IL-37 transfected HeLa cells was significantly higher than that of the empty vector control group (P<0.05), while the empty vector control group and negative control group. Conclusion The IL-37 overexpressed HeLa cell line was successfully constructed. IL-37 can inhibit proliferation and induce apoptosis of HeLa cells.关键词
IL-37/HeLa细胞/细胞增殖/细胞凋亡Key words
IL-37/HeLa cells/Cell proliferation/Apoptosis分类
医药卫生引用本文复制引用
蒋娟,邹勇德,蒋福平,陈力..IL-37对宫颈癌HeLa细胞增殖和凋亡的影响[J].肿瘤药学,2014,(4):263-266,4.
