肿瘤药学Issue(3)：179-184,6.DOI:10.3969/j.issn.2095-1264.2015.036
肌球蛋白Ⅱ对卵巢癌细胞增殖、迁移和侵袭的影响
Effects of MyosinⅡon the Proliferation, Migration and Invasion of Epithelial Ovarian Cancer Cells
摘要
Abstract
Objective To investigate the expression of MyosinⅡin epithelial ovarian cancer (EOC) and it’s effects on pro-liferation, migration and invasion in EOC cells. Methods We detected the mRNA and protein expression of MyosinⅡin the in EOC tissue specimens by real-time polymerase chain reaction (PCR) and western blot respectively. After siRNA-mediated knockdown of MyosinⅡor lentivirus-mediated overexpression of MyosinⅡin EOC cell lines, CCK-8 kits were used to detect the proliferation of EOC cells. Matrigel and Transwell were used to observe cellular migration and invasion ability. Western blot were used to detect the protein expression levels of Cyclin D1 and matrix metalloproteinase-9 (MMP-9). Results Compared with normal ovarian tissues, MyosinⅡin EOC was highly expressed and correlated with the corresponding clinical stage (P<0.05). The capacity of proliferation, migration, invasion of EOC cells with down-regulated MyosinⅡwere decreased with statisti-cally significant differences, and the protein expression levels of Cyclin D1 and MMP-9 were also decreased, as compared with the control group (P<0.001). While overexpressed MyosinⅡincreased cell proliferation, migration and invasion, as well as the protein expression levels of Cyclin D1 and MMP-9, and the differences were statistically significant as compared with the control group (P<0.001). Conclusions MyosinⅡis highly expressed in EOC and promotes the proliferation, migration and migration capability of EOC cell.关键词
肌球蛋白Ⅱ/卵巢癌细胞/增殖/迁移/侵袭Key words
MyosinⅡ/Ovarian cancer/Proliferation/Migration/Invasion分类
医药卫生引用本文复制引用
贺捷,唐洁,田文芳,唐真姿,陈亦乐,黄斯..肌球蛋白Ⅱ对卵巢癌细胞增殖、迁移和侵袭的影响[J].肿瘤药学,2015,(3):179-184,6.基金项目
国家自然科学基金项目（81101996），湖南省计划发展重点科研项目（2014FJ2015）。 （）
