肿瘤药学Issue(3)：199-204,6.DOI:10.3969/j.issn.2095-1264.2015.040
Tautomycetin与Leptomycin B联用协同抑制乳腺癌细胞增殖的作用及机制
The Role and Mechanism of Synergistic Anticancer Activity of Tautomycetin and Leptomycin B
摘要
Abstract
Objective To investigate the synergistic inhibitory effects and mechanisms of Tautomycetin (TMC) and Lep-tomycin B (LMB) on the proliferation of human breast cancer cells. Methods We investigated the synergistic effects of TMC and LMB on proliferation of breast cancer cells and expression of Bcl-2 using MTT, Western blot, cell transfection assay and fluorescence microscopy analysis. Results Our data demonstrated that the best synergistic effect was obtained by adding TMC at 24 h after treatment of LMB. The combination of TMC and LMB resulted in a reduction of half-inhibition dosage to 23.8%and 17.7%, when compared with their own control group, respectively. The combination of LMB and TMC could accelerate the down-regulation of the protein expression of Bcl-2, enhance the down-regulation of Bad, Akt and FKHR phosphoryla-tion, and promote nuclear accumulation of FKHR. Conclusion Our results indicate that the combination of TMC and LMB has a synergistic inhibitory effect on the proliferation of breast cancer cells. The synergistic effect may be associated with the enhancement of FKHR nuclear accumulation.关键词
乳腺癌/Tautomycetin/Leptomycin B/协同作用Key words
Breast cancer/Tautomycetin/Leptomycin B/Synergistic effect分类
医药卫生引用本文复制引用
刘雪娇,韩焱,许小宇,沈洋灵,牛铭山..Tautomycetin与Leptomycin B联用协同抑制乳腺癌细胞增殖的作用及机制[J].肿瘤药学,2015,(3):199-204,6.基金项目
国家自然科学基金（NO.81400167,81402074）。 （）
