中南民族大学学报（自然科学版）Issue(1)：43-46,4.
实时定量 PCR 鉴定转基因作物纯合体
Identification of the Homozygosis of Genetically Modified Crop by Real-time Quantitative PCR
摘要
Abstract
Rice endogenous reference gene phospholipase D gene ( PLD) and genetically modified ( GM) rice TT51-1 event-specific flanking sequence were used as PCR detection targets.And the homozygosis of GM rice TT51-1 plants originated from single plant seeds were analyzed through real-time PCR assays, which analyzed the Ct value of the endogenous reference gene and the flanking sequences.The reliability of this method was verified by calculation of GM copy number ratio based on the standard curves.It was concluded that the method was simple and accurate to identify the homozygosis of GM crops.关键词
转基因作物/纯合体/内标准基因/旁侧序列/实时荧光定量PCRKey words
genetically modified crops/homozygosis/endogenous reference gene/flanking sequences/real-time quantitative PCR分类
生物科学引用本文复制引用
张丽,刘丽丽,梁晓声,王海英..实时定量 PCR 鉴定转基因作物纯合体[J].中南民族大学学报（自然科学版）,2015,(1):43-46,4.基金项目
国家自然科学基金资助项目（31401607，31300829），中南民族大学中央高校基本科研业务费专项资助项目 （）
